Cationic oligonucleotides can mediate specific inhibition of gene expression in Xenopus oocytes

C P Bailey; J M Dagle; D L Weeks

doi:10.1093/nar/26.21.4860

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Cationic oligonucleotides can mediate specific inhibition of gene expression in Xenopus oocytes

Journal article

Open access

Peer reviewed

Cationic oligonucleotides can mediate specific inhibition of gene expression in Xenopus oocytes

C P Bailey, J M Dagle and D L Weeks

Nucleic acids research, Vol.26(21), pp.4860-4867

11/01/1998

DOI: 10.1093/nar/26.21.4860

PMCID: PMC147938

PMID: 9776745

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https://doi.org/10.1093/nar/26.21.4860View

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Abstract

Base-specific hydrogen bonding between an oligonucleotide and the purines in the major groove of a DNA duplex provide an approach to selective inhibition of gene expression. Oligonucleotide-mediated triplex formation in vivo may be enhanced by a number of different chemical modifications. We have previously described an in vitro analysis of triplex formation using oligonucleotides containing internucleoside phosphate linkages modified with the cation N , N -diethyl-ethylenediamine (DEED). When compared with unmodified oligonucleotides of identical base composition, DEED-modified oligonucleotides were better able to form DNA triplexes under conditions that approximate the pH, magnesium and potassium levels found in vivo . Here we report the ability of DEED-modified oligonucleotides to inhibit the expression of plasmid DNA injected into Xenopus oocytes. Inhibition is specific to plasmids containing a triplex formation target and sensitive to sequence alteration in the triplex forming target site. Inhibition of gene expression was nearly complete when oligonucleotide and plasmid were mixed together prior to injection. Inhibition was partial when oligonucleotide was injected first and not evident when plasmid was injected and allowed to form chromatin prior to oligonucleotide injection. Thus, access to DNA is a determining factor in effective triplex inhibition of gene expression.

Gene Expression - drug effects

Oocytes - metabolism

Xenopus laevis

Oligodeoxyribonucleotides - chemistry

Chloramphenicol O-Acetyltransferase - genetics

Ethylenediamines

Animals

Hydrogen Bonding

Base Sequence

Plasmids - genetics

Oocytes - drug effects

Female

Genes, Reporter - drug effects

Cations

beta-Galactosidase - genetics

In Vitro Techniques

Nucleic Acid Conformation

Oligodeoxyribonucleotides - pharmacology

Binding Sites

Details

Title: Subtitle: Cationic oligonucleotides can mediate specific inhibition of gene expression in Xenopus oocytes
Creators: C P Bailey - Department of Biochemistry and Department of Pediatrics, University of Iowa, Iowa City, IA 52242, USA
J M Dagle
D L Weeks
Resource Type: Journal article
Publication Details: Nucleic acids research, Vol.26(21), pp.4860-4867
Publisher: England
DOI: 10.1093/nar/26.21.4860
PMID: 9776745
PMCID: PMC147938
ISSN: 0305-1048
eISSN: 1362-4962
Language: English
Date published: 11/01/1998
Academic Unit: Stead Family Department of Pediatrics; Epidemiology; Biochemistry and Molecular Biology; Neonatology
Record Identifier: 9984024564702771

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