Journal article
Cav3.2 T‐type Ca++ channels trigger the endothelium‐dependent vasodilator signals activated by electrical stimulation
The FASEB journal, Vol.20(4), pp.A277-A277
03/2006
DOI: 10.1096/fasebj.20.4.A277-b
Abstract
Electrical stimulation induces vasodilator responses that spread along the vessel length without decay. We studied the transduction signals involved in the electrically induced conducted vasodilation. Mouse cremasteric arterioles were stimulated electrically (30Hz, 2ms, 30V) for 10s and the vasomotor response was evaluated at the stimulation site (local) and at 500, 1000 and 2000μm upstream. Electrical stimulation evoked local constriction and a conducted dilation. Perfusion of 10μM BAPTA‐AM for 10 min enhanced and prolonged the local constriction. Moreover, after endothelial cell BAPTA loading, the conducted dilation was delayed, smaller, and slower than control. Topical application of 10μM Ni++ or 100μM amiloride elicited vasoconstriction and blocked the electrically induced vasodilation, but not the propagation of the response, which suggests the activation of a Cav3.2‐dependent vasodilator signal. Consistent with this idea, a similar result was found in Cav3.2 knockout mice. In addition, the resting arteriolar tone and blood pressure were higher in Cav3.2 knockout mice than in controls. These results indicate that an endothelial cell Ca++ influx via activation of Cav3.2 channels triggers the vasodilator response induced by electrical stimulation. Deletion of Cav3.2 demonstrates that these channels are key in the vascular control of blood pressure. Grants: NIH #HL53318, AHA #0325730U.
Details
- Title: Subtitle
- Cav3.2 T‐type Ca++ channels trigger the endothelium‐dependent vasodilator signals activated by electrical stimulation
- Creators
- Xavier F Figueroa - University of VirginiaChien‐Chang Chen - University of IowaKevin P Campbell - University of IowaBrian R Duling - University of Virginia
- Resource Type
- Journal article
- Publication Details
- The FASEB journal, Vol.20(4), pp.A277-A277
- Publisher
- Federation of American Societies for Experimental Biology
- DOI
- 10.1096/fasebj.20.4.A277-b
- ISSN
- 0892-6638
- eISSN
- 1530-6860
- Number of pages
- 1
- Grant note
- AHA (0325730U) NIH (HL53318)
- Language
- English
- Date published
- 03/2006
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
- Record Identifier
- 9984068238202771
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