Caveolin 3 is associated with the calcium release complex and is modified via in vivo triadin modification

Stéphane Vassilopoulos; Sarah Oddoux; Séverine Groh; Marine Cacheux; Julien Fauré; Julie Brocard; Kevin P Campbell; Isabelle Marty

doi:10.1021/bi100796v

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Caveolin 3 is associated with the calcium release complex and is modified via in vivo triadin modification

Journal article

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Caveolin 3 is associated with the calcium release complex and is modified via in vivo triadin modification

Stéphane Vassilopoulos, Sarah Oddoux, Séverine Groh, Marine Cacheux, Julien Fauré, Julie Brocard, Kevin P Campbell and Isabelle Marty

Biochemistry (Easton), Vol.49(29), pp.6130-6135

07/27/2010

DOI: 10.1021/bi100796v

PMCID: PMC2907096

PMID: 20565104

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Abstract

The triadin isoforms Trisk 95 and Trisk 51 are both components of the skeletal muscle calcium release complex. To investigate the specific role of Trisk 95 and Trisk 51 isoforms in muscle physiology, we overexpressed Trisk 95 or Trisk 51 using adenovirus-mediated gene transfer in skeletal muscle of newborn mice. Overexpression of either Trisk 95 or Trisk 51 alters the muscle fiber morphology, while leaving unchanged the expression of the ryanodine receptor, the dihydropyridine receptor, and calsequestrin. We also observe an aberrant expression of caveolin 3 in both Trisk 95- and Trisk 51-overexpressing skeletal muscles. Using a biochemical approach, we demonstrate that caveolin 3 is associated with the calcium release complex in skeletal muscle. Taking advantage of muscle and non-muscle cell culture models and triadin null mouse skeletal muscle, we further dissect the molecular organization of the caveolin 3-containing calcium release complex. Our data demonstrate that the association of caveolin 3 with the calcium release complex occurs via a direct interaction with the transmembrane domain of the ryanodine receptor. Taken together, these data suggest that caveolin 3-containing membrane domains and the calcium release complex are functionally linked and that Trisk 95 and Trisk 51 are instrumental to the regulation of this interaction, the integrity of which may be crucial for muscle physiology.

Calcium - metabolism

Mice, Inbred C57BL

Muscle, Skeletal - metabolism

Ryanodine Receptor Calcium Release Channel - metabolism

Muscle, Skeletal - cytology

Mice, Knockout

Muscle Proteins - genetics

Carrier Proteins - genetics

Animals

Carrier Proteins - metabolism

Muscle Proteins - metabolism

Mice

Caveolin 3 - metabolism

Details

Title: Subtitle: Caveolin 3 is associated with the calcium release complex and is modified via in vivo triadin modification
Creators: Stéphane Vassilopoulos - INSERM U836, Grenoble Institut des Neurosciences, Equipe Muscle et Pathologies, Grenoble, France
Sarah Oddoux
Séverine Groh
Marine Cacheux
Julien Fauré
Julie Brocard
Kevin P Campbell
Isabelle Marty
Resource Type: Journal article
Publication Details: Biochemistry (Easton), Vol.49(29), pp.6130-6135
Publisher: United States
DOI: 10.1021/bi100796v
PMID: 20565104
PMCID: PMC2907096
ISSN: 0006-2960
eISSN: 1520-4995
Grant note: Howard Hughes Medical Institute
Language: English
Date published: 07/27/2010
Academic Unit: Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
Record Identifier: 9984020893502771

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