Journal article
Cdc42 Regulates Microtubule-Dependent Golgi Positioning
Traffic (Copenhagen, Denmark), Vol.11(8), pp.1067-1078
08/01/2010
DOI: 10.1111/j.1600-0854.2010.01082.x
PMCID: PMC2904418
PMID: 20525016
Abstract
The molecular mechanisms underlying cytoskeleton-dependent Golgi positioning are poorly understood. In mammalian cells, the Golgi apparatus is localized near the juxtanuclear centrosome via dynein-mediated motility along microtubules. Previous studies implicate Cdc42 in regulating dynein-dependent motility. Here we show that reduced expression of the Cdc42-specific GTPase-activating protein, ARHGAP21, inhibits the ability of dispersed Golgi membranes to reposition at the centrosome following nocodazole treatment and washout. Cdc42 regulation of Golgi positioning appears to involve ARF1 and a binding interaction with the vesicle-coat protein coatomer. We tested whether Cdc42 directly affects motility, as opposed to the formation of a trafficking intermediate, using a Golgi capture and motility assay in permeabilized cells. Disrupting Cdc42 activation or the coatomer/Cdc42 binding interaction stimulated Golgi motility. The coatomer/Cdc42-sensitive motility was blocked by the addition of an inhibitory dynein antibody. Together, our results reveal that dynein and microtubule-dependent Golgi positioning is regulated by ARF1-, coatomer-, and ARHGAP21-dependent Cdc42 signaling.
Details
- Title: Subtitle
- Cdc42 Regulates Microtubule-Dependent Golgi Positioning
- Creators
- Heidi Hehnly - Roy J. and Lucille A. Carver College of MedicineWeidong Xu - Roy J. and Lucille A. Carver College of MedicineJi-Long Chen - Current address: Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaMark Stamnes - Roy J. and Lucille A. Carver College of Medicine
- Resource Type
- Journal article
- Publication Details
- Traffic (Copenhagen, Denmark), Vol.11(8), pp.1067-1078
- DOI
- 10.1111/j.1600-0854.2010.01082.x
- PMID
- 20525016
- PMCID
- PMC2904418
- NLM abbreviation
- Traffic
- ISSN
- 1398-9219
- eISSN
- 1600-0854
- Publisher
- Wiley
- Number of pages
- 12
- Grant note
- 0950167G / American Heart Association T32HL007638 / NATIONAL HEART, LUNG, AND BLOOD INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI) R01GM068674 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) RO1 GM068674; 5 T32 HL 07638-23 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Language
- English
- Date published
- 08/01/2010
- Academic Unit
- Molecular Physiology and Biophysics; Internal Medicine
- Record Identifier
- 9984297502402771
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