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Cefazolin embedded biodegradable polypeptide nanofilms promising for infection prevention: A preliminary study on cell responses
Journal article   Open access   Peer reviewed

Cefazolin embedded biodegradable polypeptide nanofilms promising for infection prevention: A preliminary study on cell responses

Hongshuai Li, Heather Ogle, Bingbing Jiang, Michael Hagar and Bingyun Li
Journal of orthopaedic research, Vol.28(8), pp.992-999
08/2010
DOI: 10.1002/jor.21115
PMCID: PMC3699871
PMID: 20162715
url
https://doi.org/10.1002/jor.21115View
Published (Version of record) Open Access

Abstract

Implant-associated infection is a serious complication in orthopedic surgery, and endowing implant surfaces with antibacterial properties could be one of the most promising approaches for preventing such infection. In this study, we developed cefazolin loaded biodegradable polypeptide multilayer nanofilms on orthopedic implants. We found that the amount of cefazolin released could be tuned. A high local concentration of cefazolin was achieved within the first a few hours and therefore may inhibit bacterial colonization in the critical postimplantation period. The developed cefazolin loaded nanofilms showed their in vitro efficacy against Staphylococcus aureus; the more antibiotics loaded, the longer the nanocoated implant had antibacterial properties. More interestingly, antibiotic-loaded polypeptide multilayer nanofilms also improved osteoblast bioactivity including cell viability and proliferation. These findings suggested that biodegradable polypeptide multilayernanofilms as antibiotic carriers at the implant/tissue interface are compatible withhumancells such as osteoblasts and bactericidal to bacteria such as S. aureus. These characteristics could be promising for preventing implant-associated infection and potentially improving bone healing. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
antibiotic electrostatic layer-by-layer self-assembly implant-associated infection local antibiotic delivery polypeptide

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