Cell Wall Contents of Probiotics (Lactobacillus species) Protect Against Lipopolysaccharide (LPS)-Induced Murine Colitis by Limiting Immuno-inflammation and Oxidative Stress

Mehulkumar Ramanlal Chorawala; Sweta Chauhan; Rakesh Patel; Gaurang Shah

doi:10.1007/s12602-020-09738-4

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Cell Wall Contents of Probiotics (Lactobacillus species) Protect Against Lipopolysaccharide (LPS)-Induced Murine Colitis by Limiting Immuno-inflammation and Oxidative Stress

Journal article

Peer reviewed

Cell Wall Contents of Probiotics (Lactobacillus species) Protect Against Lipopolysaccharide (LPS)-Induced Murine Colitis by Limiting Immuno-inflammation and Oxidative Stress

Mehulkumar Ramanlal Chorawala, Sweta Chauhan, Rakesh Patel and Gaurang Shah

Probiotics and antimicrobial proteins, Vol.13(4), pp.1005-1017

08/2021

DOI: 10.1007/s12602-020-09738-4

PMID: 33544362

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Abstract

Currently, there are no effective therapeutic agents to limit intestinal mucosal damage associated with inflammatory bowel disease (IBD). Based on several clinical studies, probiotics have emerged as a possible novel therapeutic strategy for IBD; however, their possible mechanisms are still poorly understood. Although probiotics in murine and human improve disease severity, very little is known about the specific contribution of cell wall contents of probiotics in IBD. Herein, we investigated the protective effects of cell wall contents of three Lactobacillus species in lipopolysaccharide (LPS)-induced colitis rats. LPS-sensitized rats were rendered colitic by colonic instillation of LPS (500 µg/rat) for 14 consecutive days. Concurrently, cell wall contents isolated from 10 CFU of L. casei (LC), L. acidophilus (LA), and L. rhamnosus (LA) was given subcutaneously for 21 days, considering sulfasalazine (100 mg/kg, p.o.) as standard. The severity of colitis was assessed by body weight loss, food intake, stool consistency, rectal bleeding, colon weight/length, spleen weight, and histological analysis. Colonic inflammatory markers (myeloperoxidase activity, C-reactive protein, and pro-inflammatory cytokines) and oxidative stress markers (malondialdehyde, reduced glutathione, and nitric oxide) were also assayed. Cell wall contents of LC, LA, and LR significantly ameliorated the severity of colitis by reducing body weight loss and diarrhea and bleeding incidence, improving food intake, colon weight/length, spleen weight, and microscopic damage to the colonic mucosa. The treatment also reduced levels of inflammatory and oxidative stress markers and boosted anti-oxidant molecule. In conclusion, cell wall contents of LC, LA, and LR attenuate LPS-induced colitis by modulating immuno-inflammation and oxidative stress.

Animals

Cell Wall - chemistry

Colitis - chemically induced

Colitis - therapy

Lactobacillus

Lipopolysaccharides

Oxidative Stress

Probiotics

Rats

Weight Loss

Details

Title: Subtitle: Cell Wall Contents of Probiotics (Lactobacillus species) Protect Against Lipopolysaccharide (LPS)-Induced Murine Colitis by Limiting Immuno-inflammation and Oxidative Stress
Creators: Mehulkumar Ramanlal Chorawala - Department of Pharmacology, L. M. College of Pharmacy, Ahmedabad, Gujarat, India. mehul.chorawala@lmcp.ac.in
Sweta Chauhan - Department of Pharmacology, K. B. Institute of Pharmaceutical Education and Research, Gandhinagar, Gujarat, India
Rakesh Patel - University of Iowa
Gaurang Shah - B.J. Medical College
Resource Type: Journal article
Publication Details: Probiotics and antimicrobial proteins, Vol.13(4), pp.1005-1017
DOI: 10.1007/s12602-020-09738-4
PMID: 33544362
ISSN: 1867-1306
eISSN: 1867-1314
Language: English
Date published: 08/2021
Academic Unit: Internal Medicine
Record Identifier: 9985132184302771

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