Journal article
Cell cycle progression during chronic hyperthermia in S phase CHO cells
International journal of hyperthermia, Vol.5(3), pp.405-415
1989
DOI: 10.3109/02656738909140467
PMID: 2723477
Abstract
S phase CHO cells chronically exposed to 41.5°C exhibited an apparent resistance to further cell killing after 4-6 h. During the treatment interval, cells were observed to progress out of S phase and into G2, mitosis, and the next cell cycle. Progression through S was delayed by about 2 h and an equivalent mitotic delay was observed. After entry into mitosis, heated cultures showed an altered nuclear morphology, presumably as the result of abnormal division occurring during the treatment. Tritium suicide experiments at this temperature showed that clonogenic, as well as non-clonogenic, cells progressed during this period. When S phase cultures were chronically heated at 42°C, however, the delay in transit through S phase was 8-10 h, and an accumulation in G2 phase was observed. Therefore, our results show that, in S phase cultures heated at 42°C, cell killing continues while cells progress through S, suggesting that chronic thermotolerance cannot be expressed in S phase. Furthermore, at 41.5°C, cells which progress out of S during the treatment express resistance to heat killing in subsequent cell cycle phases. In summary, our results indicate that, although chronic tolerance is not expressed during heating in S phase, it is expressed after the cells progress out of S phase.
Details
- Title: Subtitle
- Cell cycle progression during chronic hyperthermia in S phase CHO cells
- Creators
- Michael A Mackey - 1Radiation Oncology Research Laboratory, University of California, CED-200, San Francisco, CA, 94143, USAWilliam C Dewey - 1Radiation Oncology Research Laboratory, University of California, CED-200, San Francisco, CA, 94143, USA
- Resource Type
- Journal article
- Publication Details
- International journal of hyperthermia, Vol.5(3), pp.405-415
- Publisher
- Informa UK Ltd
- DOI
- 10.3109/02656738909140467
- PMID
- 2723477
- ISSN
- 0265-6736
- eISSN
- 1464-5157
- Language
- English
- Date published
- 1989
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology
- Record Identifier
- 9984064556502771
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