Journal article
Cell diameter doesn't affect lipid productivity of Chlorococcum littorale
Algal research (Amsterdam), Vol.19, pp.333-341
11/01/2016
DOI: 10.1016/j.algal.2016.02.002
Abstract
We hypothesized that cells with different diameter have different division rates, which could affect lipid productivity (lipid content×biomass productivity). In the present work we assessed the influence of cell diameter, as a sorting parameter, on both biomass and lipid productivity of Chlorococcum littorale. Prior to sorting, cells were grown in a batch-wise nitrogen run-out including a long nitrogen depleted phase (N−) to stop cell division, thus only having vegetative cells (Pre-sorting). Cell sorting was done at the end of this N− phase using FACS (fluorescence assisted cell sorting) based on forward scatter as a proxy for diameter (size ranges (μm): 5–6 (small), 8–9 (medium), 11–14 (large) and 5–14 (control)). The sorting was done in 2 pools: multiple-cell (100 cells) and single-cell. After sorting, cells were recovered under low-light for 2weeks, and used to start the Post-sorting experiment (analogous to Pre-sorting). The populations derived from different sorted pools, single-cell and multiple-cell, showed similar size distributions after re-growth. No difference was observed in biomass and lipid productivities among Post-sorting cells and when compared to Pre-sorting cells under nitrogen depletion. We concluded that cellular size had no effect on both biomass and lipid productivity of C. littorale.
•Successful cell sorting based on light forward scatter as proxy for cell diameter.•Single and multiple-cell sorting lead to populations with similar size distribution.•Cultures started with different diameters had similar final lipid productivities.•Cell size, as sorting criterion, had no effect on biomass and lipid productivity.
Details
- Title: Subtitle
- Cell diameter doesn't affect lipid productivity of Chlorococcum littorale
- Creators
- Iago Teles Dominguez Cabanelas - Wageningen University & ResearchCarolina Fernandes - Wageningen University & ResearchDorinde M.M. Kleinegris - Wageningen University & ResearchRené H. Wijffels - Nord UniversityMaria J. Barbosa - Wageningen UR, Food & Biobased Research, AlgaePARC, Bornsesteeg 10, Building 112, 6721NG Bennekom, Netherlands
- Resource Type
- Journal article
- Publication Details
- Algal research (Amsterdam), Vol.19, pp.333-341
- DOI
- 10.1016/j.algal.2016.02.002
- ISSN
- 2211-9264
- eISSN
- 2211-9264
- Publisher
- Elsevier B.V
- Grant note
- DOI: 10.13039/501100004725, name: Ministry of Economic Affairs; name: Agriculture and Innovation; name: Province of Gelderland; DOI: 10.13039/100004349, name: BASF; name: BioOils; name: Drie Wilgen Development; name: DSM; name: Exxon Mobil; name: GEA Westfalia Separator; name: Heliae; name: Neste Oil; name: Nijhuis; name: Paques; name: Cellulac; name: Proviron; name: Roquette; DOI: 10.13039/501100007164, name: SABIC; name: Simris Alg; name: Staatsolie Suriname; name: Synthetic Genomics; DOI: 10.13039/501100007185, name: TOTAL; DOI: 10.13039/100007190, name: Unilever, award: TRCDKI/2010/0956; name: National Council of Science and Technology, award: 236614/2012-6
- Language
- English
- Date published
- 11/01/2016
- Academic Unit
- International Programs; Spanish and Portuguese
- Record Identifier
- 9984397912902771
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