Cell mediated immune responses following revaccination with an influenza A/H5N1 vaccine

Innocent N Mbawuike; Robert L Atmar; Shital M Patel; David B Corry; Patricia L Winokur; Rebecca C Brady; Wilbur H Chen; Kathryn M Edwards; C. Buddy Creech; Emmanuel B Walter; Sharon E Frey; Robert B Belshe; Johannes B Goll; Heather Hill; Wendy A Keitel

doi:10.1016/j.vaccine.2015.11.055

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Cell mediated immune responses following revaccination with an influenza A/H5N1 vaccine

Journal article

Open access

Peer reviewed

Cell mediated immune responses following revaccination with an influenza A/H5N1 vaccine

Innocent N Mbawuike, Robert L Atmar, Shital M Patel, David B Corry, Patricia L Winokur, Rebecca C Brady, Wilbur H Chen, Kathryn M Edwards, C. Buddy Creech, Emmanuel B Walter, …

Vaccine, Vol.34(4), pp.547-554

01/20/2016

DOI: 10.1016/j.vaccine.2015.11.055

PMCID: PMC4713282

PMID: 26657997

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Abstract

•CMI responses occurred following administration of an inactivated A/H5N1 vaccine.•High dose vaccine increased the magnitude of CMI response only in the unprimed subjects.•IFN-γ nSFC levels correlated with IFN-γ concentrations in PBMC supernatants. The study aims were to determine whether inactivated influenza A/H5N1 vaccine administration elicited cell mediated immune (CMI) responses and the impact of adjuvant, vaccine dose and subject age on these responses. Adults who were previously primed with either adjuvanted or unadjuvanted, inactivated, A/H5N1/Vietnam/1203/2004 (Clade 1) vaccine or unprimed (received placebo) in previous vaccine studies were randomized to receive one (primed) or two (unprimed) 15- or 90-mcg doses of inactivated, A/H5N1/Indonesia/05/05 (Clade 2) vaccine. Peripheral blood mononuclear cells (PBMCs) were collected and analyzed from a subset of vaccinees to assess CMI responses using IFN-γ and granzyme B ELISPOT assays. Cytokine measurements were performed on PBMC supernatants after stimulation with H5N1 virus. PBMCs were available from 177 participants; 88 and 89 received 15-mcg and 90-mcg of unadjuvanted clade 2 vaccine, respectively. Following H5N1 clade 1 stimulation, IFN-γ but not granzyme B normalized spot-forming cell numbers had statistically significant increased numbers at each of the post-vaccination timepoints compared to baseline in pooled analyses of all vaccine doses and age groups. Clade 2 stimulation resulted in statistically significant increased numbers of IFN-γ cells only 180 days following the last vaccination. Responses were similar among younger and older study participants, as were responses among those primed with alum-adjuvanted or non-adjuvanted clade 1 H5N1 vaccines. The dosage of clade 2 vaccine did not impact CMI responses among primed subjects, but responses were statistically significantly greater in unprimed recipients of the 90-mcg dosage compared to unprimed recipients of the 15-mcg dosage. IFN-γ levels in the supernatants of stimulated PBMC were strongly correlated with IFN-γ ELISPOT results. CMI responses occur in adults administered influenza A/H5N1 inactivated influenza vaccine.

H5N1

Adjuvant

Cell mediated immune response

Inactivated influenza vaccine

Cytokine

Details

Title: Subtitle: Cell mediated immune responses following revaccination with an influenza A/H5N1 vaccine
Creators: Innocent N Mbawuike - Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, United States
Robert L Atmar - Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, United States
Shital M Patel - Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, United States
David B Corry - Department of Medicine, Baylor College of Medicine, Houston, TX, United States
Patricia L Winokur - Department of Internal Medicine, University of Iowa, Iowa City, IA, United States
Rebecca C Brady - Gamble Program for Clinical Studies, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, United States
Wilbur H Chen - Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD, United States
Kathryn M Edwards - Vanderbilt Vaccine Research Program, Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN, United States
C. Buddy Creech - Vanderbilt Vaccine Research Program, Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN, United States
Emmanuel B Walter - Duke Clinical Vaccine Unit, Department of Pediatrics, Duke University School of Medicine, Durham, NC, United States
Sharon E Frey - Department of Internal Medicine, Saint Louis University School of Medicine, St. Louis, MO, United States
Robert B Belshe - Department of Internal Medicine, Saint Louis University School of Medicine, St. Louis, MO, United States
Johannes B Goll - EMMES Corporation, Rockville, MD, United States
Heather Hill - EMMES Corporation, Rockville, MD, United States
Wendy A Keitel - Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, United States
Resource Type: Journal article
Publication Details: Vaccine, Vol.34(4), pp.547-554
DOI: 10.1016/j.vaccine.2015.11.055
PMID: 26657997
PMCID: PMC4713282
NLM abbreviation: Vaccine
ISSN: 0264-410X
eISSN: 1873-2518
Publisher: Elsevier Ltd
Grant note: DOI: 10.13039/100007915, name: Baylor College of Medicine, award: HHSN272200800002C; DOI: 10.13039/100008893, name: University of Iowa, award: HHSN272200800008C; DOI: 10.13039/100007172, name: Cincinnati Children's Hospital, award: HHSN272200800006C; DOI: 10.13039/100008510, name: University of Maryland, award: HHSN272200800001C; DOI: 10.13039/100006537, name: Vanderbilt University, award: HHSN272200800007C; DOI: 10.13039/100006650, name: Saint Louis University, award: HHSN272200800003C; name: EMMES Corporation, award: HHSN272200800013C
Language: English
Date published: 01/20/2016
Academic Unit: Infectious Diseases; Medicine Administration; Internal Medicine
Record Identifier: 9984094576902771

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