Cell-specific effects of pentoxifylline on nitric oxide production and inducible nitric oxide synthase mRNA expression

V Trajković; V Badovinac; D Popadić; O Hadzić; M M Stojković

doi:10.1046/j.1365-2567.1997.00354.x

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Cell-specific effects of pentoxifylline on nitric oxide production and inducible nitric oxide synthase mRNA expression

Journal article

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Cell-specific effects of pentoxifylline on nitric oxide production and inducible nitric oxide synthase mRNA expression

V Trajković, V Badovinac, D Popadić, O Hadzić and M M Stojković

Immunology, Vol.92(3), pp.402-406

11/1997

DOI: 10.1046/j.1365-2567.1997.00354.x

PMCID: PMC1363803

PMID: 9486115

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Abstract

Cytokine-stimulated astrocytes and macrophages are potent producers of nitric oxide (NO), a free radical proposed to play an important role in organ-specific autoimmunity, including demyelinating diseases of the central nervous system. The aim of this study was to investigate effects of pentoxifylline (PTX), a phosphodiesterase inhibitor with immunomodulatory properties, on NO production and inducible NO synthase (iNOS) mRNA expression in rat astrocytes and macrophages. We have shown that PTX affects cytokine (interferon-gamma, IFN-gamma; interleukin-1, IL-1; tumour-necrosis factor-alpha, TNF-alpha)-induced NO production in both cell types, but in the opposite manner--enhancing in astrocytes and suppressive in macrophages. While PTX did not have any effect on enzymatic activity of iNOS in activated cells, expression of iNOS mRNA was elevated in astrocytes and decreased in macrophages treated with cytokines and PTX. Treatment with PTX alone affected neither NO production nor iNOS mRNA levels in astrocytes or macrophages. This study indicates involvement of different signalling pathways associated with iNOS induction in astrocytes and macrophages, thus emphasizing complexity of regulation of NO synthesis in different cell types.

Details

Title: Subtitle: Cell-specific effects of pentoxifylline on nitric oxide production and inducible nitric oxide synthase mRNA expression
Creators: V Trajković - Institute for Biological Research, University of Belgrade, Yugoslavia
V Badovinac - Institute for Biological Research, University of Belgrade, Yugoslavia
D Popadić - Institute for Biological Research, University of Belgrade, Yugoslavia
O Hadzić - Institute for Biological Research, University of Belgrade, Yugoslavia
M M Stojković - Institute for Biological Research, University of Belgrade, Yugoslavia
Resource Type: Journal article
Publication Details: Immunology, Vol.92(3), pp.402-406
DOI: 10.1046/j.1365-2567.1997.00354.x
PMID: 9486115
PMCID: PMC1363803
NLM abbreviation: Immunology
ISSN: 0019-2805
eISSN: 1365-2567
Language: English
Date published: 11/1997
Academic Unit: Microbiology and Immunology; Pathology
Record Identifier: 9984047995602771

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