Cell-type-specific roles of Na+/K+ ATPase subunits in Drosophila auditory mechanosensation

Madhuparna Roy; Elena Sivan-Loukianova; Daniel F Eberl

doi:10.1073/pnas.1208866110

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Cell-type-specific roles of Na+/K+ ATPase subunits in Drosophila auditory mechanosensation

Journal article

Open access

Peer reviewed

Cell-type-specific roles of Na+/K+ ATPase subunits in Drosophila auditory mechanosensation

Madhuparna Roy, Elena Sivan-Loukianova and Daniel F Eberl

Proceedings of the National Academy of Sciences - PNAS, Vol.110(1), pp.181-186

01/02/2013

DOI: 10.1073/pnas.1208866110

PMCID: PMC3538205

PMID: 23248276

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Abstract

Ion homeostasis is a fundamental cellular process particularly important in excitable cell activities such as hearing. It relies on the Na(+)/K(+) ATPase (also referred to as the Na pump), which is composed of a catalytic α subunit and a β subunit required for its transport to the plasma membrane and for regulating its activity. We show that α and β subunits are expressed in Johnston's organ (JO), the Drosophila auditory organ. We knocked down expression of α subunits (ATPα and α-like) and β subunits (nrv1, nrv2, and nrv3) individually in JO with UAS/Gal4-mediated RNAi. ATPα shows elevated expression in the ablumenal membrane of scolopale cells, which enwrap JO neuronal dendrites in endolymph-like compartments. Knocking down ATPα, but not α-like, in the entire JO or only in scolopale cells using specific drivers, resulted in complete deafness. Among β subunits, nrv2 is expressed in scolopale cells and nrv3 in JO neurons. Knocking down nrv2 in scolopale cells blocked Nrv2 expression, reduced ATPα expression in the scolopale cells, and caused almost complete deafness. Furthermore, knockdown of either nrv2 or ATPα specifically in scolopale cells causes abnormal, electron-dense material accumulation in the scolopale space. Similarly, nrv3 functions in JO but not in scolopale cells, suggesting neuron specificity that parallels nrv2 scolopale cell-specific support of the catalytic ATPα. Our studies provide an amenable model to investigate generation of endolymph-like extracellular compartments.

Glycoproteins - genetics

Immunohistochemistry

Microscopy, Electron, Transmission

Sodium-Potassium-Exchanging ATPase - genetics

Acoustic Stimulation

Glycoproteins - metabolism

Drosophila Proteins - metabolism

Mechanotransduction, Cellular - physiology

Nerve Tissue Proteins - genetics

Drosophila - physiology

Protein Subunits - metabolism

Nerve Tissue Proteins - metabolism

Microscopy, Confocal

Sodium-Potassium-Exchanging ATPase - metabolism

Animals

RNA Interference

Homeostasis - physiology

Hearing - physiology

Drosophila Proteins - genetics

Details

Title: Subtitle: Cell-type-specific roles of Na+/K+ ATPase subunits in Drosophila auditory mechanosensation
Creators: Madhuparna Roy - Department of Biology, The University of Iowa, Iowa City, IA 52242, USA
Elena Sivan-Loukianova
Daniel F Eberl
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.110(1), pp.181-186
Publisher: United States
DOI: 10.1073/pnas.1208866110
PMID: 23248276
PMCID: PMC3538205
ISSN: 0027-8424
eISSN: 1091-6490
Grant note: DC004848 / NIDCD NIH HHS R01 DC004848 / NIDCD NIH HHS P30 DC010362 / NIDCD NIH HHS
Language: English
Date published: 01/02/2013
Academic Unit: Iowa Neuroscience Institute; Biology
Record Identifier: 9983991959802771

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