Cell viability viscoelastic measurement in a rheometer used to stress and engineer tissues at low sonic frequencies

Sarah A Klemuk; Sanyukta Jaiswal; Ingo R Titze

doi:10.1121/1.2973183

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Cell viability viscoelastic measurement in a rheometer used to stress and engineer tissues at low sonic frequencies

Journal article

Peer reviewed

Cell viability viscoelastic measurement in a rheometer used to stress and engineer tissues at low sonic frequencies

Sarah A Klemuk, Sanyukta Jaiswal and Ingo R Titze

The Journal of the Acoustical Society of America, Vol.124(4), pp.2330-2339

10/01/2008

DOI: 10.1121/1.2973183

PMCID: PMC2677341

PMID: 19062871

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Abstract

Effects of vibration on human vocal fold extracellular matrix composition and the resultant tissue viscoelastic properties are difficult to study in vivo. Therefore, an in vitro bioreactor, simulating the in vivo physiological environment, was explored. A stress-controlled commercial rheometer was used to administer shear vibrations to living tissues at stresses and frequencies corresponding to male phonation, while simultaneously measuring tissue viscoelastic properties. Tissue environment was evaluated and adjustments made in order to sustain cell life for short term experimentation up to 6 h. Cell nutrient medium evaporation, osmolality, pH, and cell viability of cells cultured in three-dimensional synthetic scaffolds were quantified under comparably challenging environments to the rheometer bioreactor for 4 or 6 h. The functionality of the rheometer bioreactor was demonstrated by applying three vibration regimes to cell-seeded three-dimensional substrates for 2 h. Resulting strain was quantified throughout the test period. Rheologic data and cell viability are reported for each condition, and future improvements are discussed.

Bioreactors

Cell Line

Cell Survival

Elasticity

Extracellular Matrix - physiology

Fibroblasts - physiology

Humans

Hydrogen-Ion Concentration

Male

Models, Biological

Osmolar Concentration

Phonation

Stress, Mechanical

Time Factors

Tissue Scaffolds

Torque

Ultrasonics

Vibration

Viscosity

Vocal Cords - physiology

Details

Title: Subtitle: Cell viability viscoelastic measurement in a rheometer used to stress and engineer tissues at low sonic frequencies
Creators: Sarah A Klemuk - University of Iowa
Sanyukta Jaiswal - University of Iowa
Ingo R Titze - University of Iowa
Resource Type: Journal article
Publication Details: The Journal of the Acoustical Society of America, Vol.124(4), pp.2330-2339
DOI: 10.1121/1.2973183
PMID: 19062871
PMCID: PMC2677341
NLM abbreviation: J Acoust Soc Am
ISSN: 0001-4966
eISSN: 1520-8524
Grant note: R01 DC004224 / NIDCD NIH HHS DC008047 / NIDCD NIH HHS R01 DC004224-07 / NIDCD NIH HHS DC004224 / NIDCD NIH HHS R01 DC004224-08 / NIDCD NIH HHS F31 DC008047 / NIDCD NIH HHS
Language: English
Date published: 10/01/2008
Academic Unit: School of Music; Communication Sciences and Disorders
Record Identifier: 9984719571802771

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