Journal article
Cellular Proteins Bind to Sequence Motifs in the R1 Element between the HCMV Immune Evasion Genes
Experimental and molecular pathology, Vol.72(3), pp.196-206
06/01/2002
DOI: 10.1006/exmp.2002.2428
PMID: 12009783
Abstract
The viral US3 and US6 gene products of human cytomegalovirus (CMV) are sequentially expressed at immediate-early and early times after infection, respectively. They downregulate the surface expression of HLA class I molecules. There are two repeat-containing regulatory regions between the US3 promoter and the US6 transcription unit designated R1 and R2. R2 contains repetitions of the NF-κB responsive element and enhances the immediate-early expression of the US3 gene. R1 contains 19 repetitions of a 5′-TRTCG-3′ pentanucleotide arranged as everted repeats, inverted repeats, and variably spaced single pentanucleotides. In the context of the viral genome, R1 also enhances immediate-early US3 gene expression by an unknown mechanism (G. C. Bullock,
et al., 2001,
Virology 288, 164–174). We report a sequence motif within the R1 element that binds a human cell nuclear protein which is antigenically related to the
Drosophila boundary element-associated factor (BEAF). The potential role of a 35-kDa cellular protein that binds to sequence motifs within the R1 element in regulating the expression of the CMV US3 immune evasion gene is discussed.
Details
- Title: Subtitle
- Cellular Proteins Bind to Sequence Motifs in the R1 Element between the HCMV Immune Evasion Genes
- Creators
- Grant C. Bullock - University of IowaAbby R. Thrower - Program in Genetics, College of Medicine, University of Iowa, Iowa City, Iowa, 52242Mark F. Stinski - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Experimental and molecular pathology, Vol.72(3), pp.196-206
- Publisher
- Elsevier Inc
- DOI
- 10.1006/exmp.2002.2428
- PMID
- 12009783
- ISSN
- 0014-4800
- eISSN
- 1096-0945
- Language
- English
- Date published
- 06/01/2002
- Academic Unit
- Microbiology and Immunology; Pathology
- Record Identifier
- 9984697738802771
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