Journal article
Cellular differentiation, bioactive and mechanical properties of experimental light-curing pulp protection materials
Dental materials, Vol.34(6), pp.868-878
06/2018
DOI: 10.1016/j.dental.2018.02.008
PMID: 29555096
Abstract
[Display omitted]
•Incorporation of bioactive fillers into resin-based materials induces apatite precipitation.•Resins containing zinc-doped bioactive fillers maintain stable mechanical properties over time.•Zinc-doped bioactive fillers have excellent osteo-inductivity/dentinogenesis and biocompatibility properties.
Materials for pulp protection should have therapeutic properties in order to stimulate remineralization and pulp reparative processes. The aim of this study was to evaluate the mechanical properties, biocompatibility, cell differentiation and bioactivity of experimental light-curable resin-based materials containing bioactive micro-fillers.
Four calcium-phosphosilicate micro-fillers were prepared and incorporated into a resin blend: 1) Bioglass 45S5 (BAG); 2) zinc-doped bioglass (BAG-Zn); 3) βTCP-modified calcium silicate (β-CS); 4) zinc-doped β-CS (β-CS-Zn). These experimental resins were tested for flexural strength (FS) and fracture toughness (FT) after 24h and 30-day storage in simulated body fluid (SBF). Cytotoxicity was evaluated using MTT assay, while bioactivity was evaluated using mineralization and gene expression assays (Runx-2 & ALP).
The lowest FS and FT at 24h was attained with β-CS resin, while all the other tested materials exhibited a decrease in FS after prolonged storage in SBF. β-CS-Zn maintained a stable FT after 30-day SBF aging. Incorporation of bioactive micro-fillers had no negative effect on the biocompatibility of the experimental materials tested in this study. The inclusion of zinc-doped fillers significantly increased the cellular remineralization potential and expression of the osteogenic genes Runx2 and ALP (p<0.05).
The innovative materials tested in this study, in particular those containing β-CS-Zn and BAG-Zn may promote cell differentiation and mineralization. Thus, these materials might represent suitable therapeutic pulp protection materials for minimally invasive and atraumatic restorative treatments.
Details
- Title: Subtitle
- Cellular differentiation, bioactive and mechanical properties of experimental light-curing pulp protection materials
- Creators
- Salvatore Sauro - Universidad Cardenal Herrera CEUAshvin Babbar - King's College LondonBorzo Gharibi - King's College LondonVictor Pinheiro Feitosa - Universidade Federal do CearáRicardo Marins Carvalho - University of British ColumbiaLidiany Karla Azevedo Rodrigues - School of Dentistry, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, BrazilAvijit Banerjee - King's College LondonTimothy Watson - Kings Health Partners
- Resource Type
- Journal article
- Publication Details
- Dental materials, Vol.34(6), pp.868-878
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.dental.2018.02.008
- PMID
- 29555096
- ISSN
- 0109-5641
- eISSN
- 1879-0097
- Language
- English
- Date published
- 06/2018
- Academic Unit
- Operative Dentistry
- Record Identifier
- 9984654469902771
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