Cellular factors are required to activate bovine papillomavirus-1 early gene transcription and to establish viral plasmid persistence but are not required for cellular transformation

Thomas H Haugen; Michael J Lace; Takaoki Ishiji; Atsushi Sameshima; James R Anson; Lubomir P Turek

doi:10.1016/j.virol.2009.04.002

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Cellular factors are required to activate bovine papillomavirus-1 early gene transcription and to establish viral plasmid persistence but are not required for cellular transformation

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Cellular factors are required to activate bovine papillomavirus-1 early gene transcription and to establish viral plasmid persistence but are not required for cellular transformation

Thomas H Haugen, Michael J Lace, Takaoki Ishiji, Atsushi Sameshima, James R Anson and Lubomir P Turek

Virology (New York, N.Y.), Vol.389(1), pp.82-90

2009

DOI: 10.1016/j.virol.2009.04.002

PMID: 19410271

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Abstract

Transcription from the major upstream early gene promoter, P89, of bovine papillomavirus (BPV)-1 is detectable in transfected cells lacking viral gene products yet also responds to viral E2 proteins. In contrast to human papillomaviruses (HPVs), the BPV upstream regulatory region (URR) functions as a transcriptional enhancer in epithelial cells and fibroblasts of bovine, murine or human origin. Mutations of Sp1 and/or two novel transcriptional enhancer factor (TEF)-1 sites within the 5′ URR of the intact BPV-1 genome dramatically reduced P89-initiated mRNA levels, leading to decreased BPV-1 plasmid amplification and inefficient formation of transformed cell foci. However, cell lines transformed with wt or mutant BPV-1 genomes contained similar levels of unintegrated BPV-1 DNA, P89 mRNA and E2-dependent transactivation. We conclude that cellular factors necessary for activating viral early gene transcription, establishment of viral plasmid replication and cell immortalization are not required during the maintenance phase of BPV-1 infection.

BPV replication

Transformation

Transcription

Early gene expression

Details

Title: Subtitle: Cellular factors are required to activate bovine papillomavirus-1 early gene transcription and to establish viral plasmid persistence but are not required for cellular transformation
Creators: Thomas H Haugen - Veterans Affairs Medical Center, The Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
Michael J Lace - Veterans Affairs Medical Center, The Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
Takaoki Ishiji - Department of Dermatology, The Jikei University School of Medicine, Tokyo, Japan
Atsushi Sameshima - Department of Otolaryngology, Kagoshima University School of Medicine, Kagoshima, Japan
James R Anson - Veterans Affairs Medical Center, The Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
Lubomir P Turek - Veterans Affairs Medical Center, The Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Virology (New York, N.Y.), Vol.389(1), pp.82-90
DOI: 10.1016/j.virol.2009.04.002
PMID: 19410271
NLM abbreviation: Virology
ISSN: 0042-6822
eISSN: 1096-0341
Publisher: Elsevier Inc
Language: English
Date published: 2009
Academic Unit: Pathology
Record Identifier: 9984047651302771

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