Journal article
Cellular protein synthesis and inhibition of cell division are independent of butyrate-induced histone hyperacetylation
Nature (London), Vol.280(5724), pp.692-693
08/1979
DOI: 10.1038/280692a0
PMID: 471049
Abstract
SODIUM BUTYRATE has many effects on mammalian cells-inducing the biosynthesis of new proteins1-6, changing the relative synthetic rates of specific proteins7-10, increasing various enzyme activities7,9-13, inducing morphologic changes in cultured cells 9,12,14-19, and inhibiting DNA synthesis and cell division 14,15,20. The mechanism of these effects is not known. Sodium butyrate induces massive hyperacetylation of histone in cultured mammalian cells21 via inhibition of histone deacetylase22-24: this hyperacetylation can be mimicked to varying degrees by other salts of short-chain fatty acids22. As histones interact so intimately with DNA, and as the change in extent of histone acetylation is so great, we sought to determine whether the changes in histone modification are involved in causing the changes in synthesis of specific proteins or the cessation of DNA synthesis and cell division. In hepatoma tissue culture (HTC) cells, the increased amount of histone acetylation is maximal 6-12 h after administration of sodium butyrate22, and we have used two-dimensional gel techniques 25 to establish whether different proteins are made during this period. Our results suggest that cellular protein synthesis and inhibition of cell division are independent of butyrate-induced hyperacetylation. © 1979 Nature Publishing Group.
Details
- Title: Subtitle
- Cellular protein synthesis and inhibition of cell division are independent of butyrate-induced histone hyperacetylation
- Creators
- PETER Rubenstein - University of IowaLINDA Sealy - University of IowaSTEVEN Marshall - University of IowaROGER Chalkley - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Nature (London), Vol.280(5724), pp.692-693
- DOI
- 10.1038/280692a0
- PMID
- 471049
- ISSN
- 0028-0836
- eISSN
- 1476-4687
- Language
- English
- Date published
- 08/1979
- Academic Unit
- Orthodontics; Stead Family Department of Pediatrics; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984288730302771
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