Journal article
Central interactions of aldosterone and angiotensin II in aldosterone- and angiotensin II-induced hypertension
American journal of physiology. Heart and circulatory physiology, Vol.300(2), pp.H555-H564
02/2011
DOI: 10.1152/ajpheart.00847.2010
PMCID: PMC3044048
PMID: 21112947
Abstract
Many studies have implicated both angiotensin II (ANG II) and aldosterone (Aldo) in the pathogenesis of hypertension, the progression of renal injury, and cardiac remodeling after myocardial infarction. In several cases, ANG II and Aldo have been shown to have synergistic interactions in the periphery. In the present studies, we tested the hypothesis that ANG II and Aldo interact centrally in Aldo- and ANG II-induced hypertension in male rats. In rats with blood pressure (BP) and heart rate (HR) measured by DSI telemetry, intracerebroventricular (icv) infusions of the mineralocorticoid receptor (MR) antagonists spironolactone and RU28318 or the angiotensin type 1 receptor (AT
1
R) antagonist irbesartan significantly inhibited Aldo-induced hypertension. In ANG II-induced hypertension, icv infusion of RU28318 significantly reduced the increase in BP. Moreover, icv infusions of the reactive oxygen species (ROS) scavenger tempol or the NADPH oxidase inhibitor apocynin attenuated Aldo-induced hypertension. To confirm these effects of pharmacological antagonists, icv injections of either recombinant adeno-associated virus carrying siRNA silencers of AT
1a
R (AT
1a
R-siRNA) or MR (MR-siRNA) significantly attenuated the development of Aldo-induced hypertension. The immunohistochemical and Western blot analyses of AT
1a
R-siRNA- or MR-siRNA-injected rats showed a marked reduction in the expression of AT
1
R or MR in the paraventricular nucleus compared with scrambled siRNA rats. When animals from all studies underwent ganglionic blockade with hexamethonium, there was a smaller reduction in the fall of BP in animals receiving icv AT
1
R or MR antagonists. These results suggest that ANG II and Aldo interact in the brain in a mutually cooperative manner such that the functional integrity of both brain AT
1
R and MR are necessary for hypertension to be induced by either systemic ANG II or Aldo. The pressor effects produced by systemic ANG II or Aldo involve increased central ROS and sympathetic outflow.
Details
- Title: Subtitle
- Central interactions of aldosterone and angiotensin II in aldosterone- and angiotensin II-induced hypertension
- Creators
- Baojian Xue - Departments of PsychologyTerry G Beltz - Departments of PsychologyYang Yu - Internal Medicine andFang Guo - Departments of PsychologyCelso E Gomez-Sanchez - Division of Endocrinology, G. V. (Sonny) Montgomery Veterans Affairs Medical Center, University of Mississippi Medical Center, Jackson, Mississippi; andMeredith Hay - Department of Physiology, University of Arizona, Tucson, ArizonaAlan Kim Johnson - Cardiovascular Center, University of Iowa, Iowa City, Iowa
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Heart and circulatory physiology, Vol.300(2), pp.H555-H564
- DOI
- 10.1152/ajpheart.00847.2010
- PMID
- 21112947
- PMCID
- PMC3044048
- NLM abbreviation
- Am J Physiol Heart Circ Physiol
- ISSN
- 0363-6135
- eISSN
- 1522-1539
- Publisher
- American Physiological Society; Bethesda, MD
- Language
- English
- Date published
- 02/2011
- Academic Unit
- Psychological and Brain Sciences; Iowa Neuroscience Institute; Neuroscience and Pharmacology; Neurology (Pediatrics); Health, Sport, and Human Physiology ; Internal Medicine
- Record Identifier
- 9984002446702771
Metrics
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