Journal article
Centriole and Golgi microtubule nucleation are dispensable for the migration of human neutrophil-like cells
Molecular biology of the cell, Vol.32(17), pp.1545-1556
08/15/2021
DOI: 10.1091/mbc.E21-02-0060
PMCID: PMC8351748
PMID: 34191538
Abstract
Neutrophils migrate in response to chemoattractants to mediate host defense. Chemoattractants drive rapid intracellular cytoskeletal rearrangements including the radiation of microtubules from the microtubule-organizing center (MTOC) toward the rear of polarized neutrophils. Microtubules regulate neutrophil polarity and motility, but little is known about the specific role of MTOCs. To characterize the role of MTOCs on neutrophil motility, we depleted centrioles in a well-established neutrophil-like cell line. Surprisingly, both chemical and genetic centriole depletion increased neutrophil speed and chemotactic motility, suggesting an inhibitory role for centrioles during directed migration. We also found that depletion of both centrioles and GM130-mediated Golgi microtubule nucleation did not impair neutrophil directed migration. Taken together, our findings demonstrate an inhibitory role for centrioles and a resilient MTOC system in motile human neutrophil-like cells.
Details
- Title: Subtitle
- Centriole and Golgi microtubule nucleation are dispensable for the migration of human neutrophil-like cells
- Creators
- Lucas C. Klemm - University of Wisconsin–MadisonRyan A. Denu - University of Wisconsin–MadisonLaurel E. Hind - University of Wisconsin–MadisonBriana L. Rocha-Gregg - University of Wisconsin–MadisonMark E. Burkard - University of Wisconsin–MadisonAnna Huttenlocher - University of Wisconsin–Madison
- Resource Type
- Journal article
- Publication Details
- Molecular biology of the cell, Vol.32(17), pp.1545-1556
- Publisher
- The American Society for Cell Biology
- DOI
- 10.1091/mbc.E21-02-0060
- PMID
- 34191538
- PMCID
- PMC8351748
- ISSN
- 1059-1524
- eISSN
- 1939-4586
- Number of pages
- 12
- Language
- English
- Date published
- 08/15/2021
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984701258702771
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