Journal article
Centriolin Anchoring of Exocyst and SNARE Complexes at the Midbody Is Required for Secretory-Vesicle-Mediated Abscission
Cell (Cambridge), Vol.123(1), pp.75-87
2005
DOI: 10.1016/j.cell.2005.07.027
PMID: 16213214
Abstract
The terminal step in cytokinesis, called abscission, requires resolution of the membrane connection between two prospective daughter cells. Our previous studies demonstrated that the coiled-coil protein centriolin localized to the midbody during cytokinesis and was required for abscission. Here we show that centriolin interacts with proteins of vesicle-targeting exocyst complexes and vesicle-fusion SNARE complexes. These complexes require centriolin for localization to a unique midbody-ring structure, and disruption of either complex inhibits abscission. Exocyst disruption induces accumulation of v-SNARE-containing vesicles at the midbody ring. In control cells, these v-SNARE vesicles colocalize with a GFP-tagged secreted polypeptide. The vesicles move to the midbody ring asymmetrically from one prospective daughter cell; the GFP signal is rapidly lost, suggesting membrane fusion; and subsequently the cell cleaves at the site of vesicle delivery/fusion. We propose that centriolin anchors protein complexes required for vesicle targeting and fusion and integrates membrane-vesicle fusion with abscission.
Details
- Title: Subtitle
- Centriolin Anchoring of Exocyst and SNARE Complexes at the Midbody Is Required for Secretory-Vesicle-Mediated Abscission
- Creators
- Adam Gromley - Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester, Massachusetts 01605, University of Iowa, Iowa City, Iowa 52242Charles Yeaman - Department of Anatomy and Cell Biology, University of Iowa, Iowa City, Iowa 52242Jack Rosa - Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester, Massachusetts 01605, University of Iowa, Iowa City, Iowa 52242Sambra Redick - Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester, Massachusetts 01605, University of Iowa, Iowa City, Iowa 52242Chun-Ting Chen - Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester, Massachusetts 01605, University of Iowa, Iowa City, Iowa 52242Stephanie Mirabelle - Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester, Massachusetts 01605, University of Iowa, Iowa City, Iowa 52242Minakshi Guha - Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester, Massachusetts 01605, University of Iowa, Iowa City, Iowa 52242James Sillibourne - Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester, Massachusetts 01605, University of Iowa, Iowa City, Iowa 52242Stephen J Doxsey - Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester, Massachusetts 01605, University of Iowa, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- Cell (Cambridge), Vol.123(1), pp.75-87
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.cell.2005.07.027
- PMID
- 16213214
- ISSN
- 0092-8674
- eISSN
- 1097-4172
- Language
- English
- Date published
- 2005
- Academic Unit
- Anatomy and Cell Biology; Internal Medicine
- Record Identifier
- 9984025450702771
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