Journal article
Cerebral microvascular dysfunction in metabolic syndrome is exacerbated by ischemia–reperfusion injury
BMC neuroscience, Vol.18(1), pp.67-67
09/08/2017
DOI: 10.1186/s12868-017-0384-x
PMCID: PMC5591496
PMID: 28886695
Abstract
Background
Metabolic syndrome (MetS) is associated with an increased risk of cerebrovascular diseases, including cerebral ischemia. Microvascular dysfunction is an important feature underlying the pathophysiology of cerebrovascular diseases. In this study, we aimed to investigate the impacts of ischemia and reperfusion (IR) injury on the cerebral microvascular function of rats with high-fat diet-induced MetS.
Results
We examined Wistar rats fed a high-fat diet (HFD) or normal diet (CTL) for 20 weeks underwent 30 min of bilateral carotid artery occlusion followed by 1 h of reperfusion (IR) or sham surgery. Microvascular blood flow was evaluated on the parietal cortex surface through a cranial window by laser speckle contrast imaging, functional capillary density, endothelial function and endothelial–leukocyte interactions by intravital videomicroscopy. Lipid peroxidation was assessed by TBARs analysis, the expression of oxidative enzymes and inflammatory markers in the brain tissue was analyzed by real-time PCR. The cerebral IR in MetS animals induced a functional capillary rarefaction (HFD IR 117 ± 17 vs. CTL IR 224 ± 35 capillary/mm2; p < 0.05), blunted the endothelial response to acetylcholine (HFD IR −16.93% vs. CTL IR 16.19% from baseline inner diameter p < 0.05) and increased the endothelial–leukocyte interactions in the venules in the brain. The impact of ischemia on the cerebral microvascular blood flow was worsened in MetS animals, with a marked reduction of cerebral blood flow, exposing brain tissue to a higher state of hypoxia.
Conclusions
Our results demonstrate that during ischemia and reperfusion, animals with MetS are more susceptible to alterations in the cerebral microcirculation involving endothelial dysfunction and oxidative stress events.
Details
- Title: Subtitle
- Cerebral microvascular dysfunction in metabolic syndrome is exacerbated by ischemia–reperfusion injury
- Creators
- Nathalie Obadia - Fundação Oswaldo CruzMarcos Adriano Lessa - University of Iowa, AnesthesiaAnissa Daliry - Fundação Oswaldo CruzRaquel Rangel Silvares - Fundação Oswaldo CruzFabiana Gomes - Avenida Brasil, 4365, Rio de Janeiro, RJ 21045-900 BrazilEduardo Tibiriçá - Instituto Nacional de CardiologiaVanessa Estato - Fundação Oswaldo Cruz
- Resource Type
- Journal article
- Publication Details
- BMC neuroscience, Vol.18(1), pp.67-67
- Publisher
- BioMed Central
- DOI
- 10.1186/s12868-017-0384-x
- PMID
- 28886695
- PMCID
- PMC5591496
- ISSN
- 1471-2202
- eISSN
- 1471-2202
- Language
- English
- Date published
- 09/08/2017
- Academic Unit
- Anesthesia
- Record Identifier
- 9984656540302771
Metrics
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