Journal article
Cerebral vascular dysfunction mediated by superoxide in hyperhomocysteinemic mice
Stroke (1970), Vol.35(8), pp.1957-1962
2004
DOI: 10.1161/01.STR.0000131749.81508.18
PMID: 15178827
Abstract
Background and purpose: Hyperhomocysteinemia is an emerging risk factor for stroke, but little is known about effects of hyperhomocysteinemia on cerebral vascular function. We tested the hypothesis that chronic hyperhomocysteinemia in mice causes endothelial dysfunction in cerebral arterioles through a mechanism that involves superoxide.
Methods: Mice heterozygous for a targeted disruption of the cystathionine beta-synthase gene (Cbs+/-) and their wild type littermates (Cbs+/+) were fed either a control diet or a high-methionine diet for 10 to 12 months.
Results: Plasma total homocysteine was elevated with the high-methionine diet compared with the control diet for both Cbs+/+ (7.9+/-1.0 versus 5.0+/-0.5 micromol/L; P<0.05) and Cbs+/- (20.5+/-3.1 versus 8.2+/-0.9 micromol/L; P<0.001) mice. Dilatation of cerebral arterioles ( approximately 30 microm baseline diameter) was measured in vivo in response to the endothelium-dependent dilator acetylcholine or the endothelium-independent dilator nitroprusside. Vasodilatation to acetylcholine was impaired with the high-methionine diet compared with the control diet for both Cbs+/+ and Cbs+/- mice (P<0.01). Dilatation of arterioles to acetylcholine was restored toward normal by the superoxide scavenger tiron (P<0.05). Vasodilatation to nitroprusside was not influenced by Cbs genotype or diet. Dihydroethidium (DHE) staining for vascular superoxide was elevated in Cbs+/- mice fed the high-methionine diet and was inhibited by apocynin or Nomega-nitro-l-arginine methyl ester, implicating NAD(P)H oxidase and nitric oxide synthase as potential sources of superoxide.
Conclusions: Superoxide is a key mediator of endothelial dysfunction in the cerebral circulation during diet-induced hyperhomocysteinemia.
Details
- Title: Subtitle
- Cerebral vascular dysfunction mediated by superoxide in hyperhomocysteinemic mice
- Creators
- Sanjana DAYAL - Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, Iowa, United StatesErland ARNING - Baylor Institute of Metabolic Disease, Dallas, Texas, GermanyTeodoro BOTTIGLIERI - Baylor Institute of Metabolic Disease, Dallas, Texas, GermanyRainer H BÖGER - Institute of Experimental and Clinical Pharmacology and Toxicology, University Hospital Hamburg-Eppendorf, Hamburg, GermanyCurt D SIGMUND - Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, Iowa, United StatesFrank M FARACI - Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, Iowa, United StatesSteven R LENTZ - Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, Iowa, United States
- Resource Type
- Journal article
- Publication Details
- Stroke (1970), Vol.35(8), pp.1957-1962
- Publisher
- Lippincott Williams & Wilkins; Hagerstown, MD
- DOI
- 10.1161/01.STR.0000131749.81508.18
- PMID
- 15178827
- ISSN
- 0039-2499
- eISSN
- 1524-4628
- Language
- English
- Date published
- 2004
- Academic Unit
- Molecular Physiology and Biophysics; Hematology, Oncology, and Blood & Marrow Transplantation; Iowa Neuroscience Institute; Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984040386002771
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