Cerebroglycan, a developmentally regulated cell-surface heparan sulfate proteoglycan, is expressed on developing axons and growth cones

Jonathan K Ivins; E David Litwack; Asli Kumbasar; Christopher S Stipp; Arthur D Lander

doi:10.1006/dbio.1997.8532

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Cerebroglycan, a developmentally regulated cell-surface heparan sulfate proteoglycan, is expressed on developing axons and growth cones

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Cerebroglycan, a developmentally regulated cell-surface heparan sulfate proteoglycan, is expressed on developing axons and growth cones

Jonathan K Ivins, E David Litwack, Asli Kumbasar, Christopher S Stipp and Arthur D Lander

Developmental biology, Vol.184(2), pp.320-332

04/15/1997

DOI: 10.1006/dbio.1997.8532

PMID: 9133438

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Abstract

Cerebroglycan is a glycosylphosphatidylinositol-linked integral membrane heparan sulfate proteoglycan found exclusively in the developing nervous system. In the rodent, cerebroglycan mRNA first appears in regions containing newly generated neurons and typically disappears 1 to several days later (Stipp et al., 1994, J. Cell Biol. 124:149-160). To gain insight into the roles that cerebroglycan plays in the developing nervous system, monospecific antibodies were prepared and used to localize cerebroglycan protein. In the rat, cerebroglycan was prominantly expressed on axon tracts throughout the developing brain and spinal cord, where it was found at times when axons are actively growing, but generally not after axons have reached their targets. Cerebroglycan was also found on neuronal growth cones both in vivo and in vitro. Interestingly, cerebroglycan immunoreactivity was rarely seen in or around neuronal cell bodies. Indeed, by examining the hippocampus at a late stage in development-when most neurons no longer express cerebroglycan but newly generated granule neurons do-evidence was obtained that cerebroglycan is strongly polarized to the axonal, and excluded from the somatodendritic, compartment of neurons. The timing and pattern of cerebroglycan expression are consistent with a role for this cell-surface heparan sulfate proteoglycan in regulating the growth or guidance of axons.

Immunochemistry

Polysaccharide-Lyases - metabolism

Brain - embryology

Spinal Cord - metabolism

Neurons - cytology

Axons - physiology

RNA, Messenger - metabolism

Heparitin Sulfate - metabolism

PC12 Cells

Membrane Proteins - analysis

Brain - metabolism

Glypicans

Dentate Gyrus - embryology

In Situ Hybridization

Gene Expression Regulation, Developmental

Membrane Proteins - physiology

Membrane Proteins - metabolism

Dentate Gyrus - metabolism

Neurons - chemistry

Chondroitin Lyases - metabolism

Membrane Proteins - genetics

Cells, Cultured

Proteoglycans - metabolism

Rats

Axons - chemistry

Proteoglycans - analysis

Rats, Sprague-Dawley

Blotting, Western

Spinal Cord - embryology

Animals

Membrane Proteins - chemistry

Proteoglycans - physiology

Heparan Sulfate Proteoglycans

Proteoglycans - genetics

Details

Title: Subtitle: Cerebroglycan, a developmentally regulated cell-surface heparan sulfate proteoglycan, is expressed on developing axons and growth cones
Creators: Jonathan K Ivins - Department of Cell and Developmental Biology, University of California at Irvine, 92697, USA. jkivins@UCI.edu
E David Litwack
Asli Kumbasar
Christopher S Stipp
Arthur D Lander
Resource Type: Journal article
Publication Details: Developmental biology, Vol.184(2), pp.320-332
DOI: 10.1006/dbio.1997.8532
PMID: 9133438
NLM abbreviation: Dev Biol
ISSN: 0012-1606
eISSN: 1095-564X
Publisher: United States
Grant note: NS26862 / NINDS NIH HHS
Language: English
Date published: 04/15/1997
Academic Unit: Molecular Physiology and Biophysics; Biology
Record Identifier: 9983991962302771

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