Cerebroglycan: an integral membrane heparan sulfate proteoglycan that is unique to the developing nervous system and expressed specifically during neuronal differentiation

Christopher S Stipp; E David Litwack; Arthur D Lander

doi:10.1083/jcb.124.1.149

Back

Cerebroglycan: an integral membrane heparan sulfate proteoglycan that is unique to the developing nervous system and expressed specifically during neuronal differentiation

Journal article

Open access

Peer reviewed

Cerebroglycan: an integral membrane heparan sulfate proteoglycan that is unique to the developing nervous system and expressed specifically during neuronal differentiation

Christopher S Stipp, E David Litwack and Arthur D Lander

The Journal of cell biology, Vol.124(1-2), pp.149-160

01/1994

DOI: 10.1083/jcb.124.1.149

PMID: 8294498

Files and links (1)

url

https://doi.org/10.1083/jcb.124.1.149View

Published (Version of record) Open Access

Abstract

Heparan sulfate proteoglycans (HSPGs) are found on the surface of all adherent cells and participate in the binding of growth factors, extracellular matrix glycoproteins, cell adhesion molecules, and proteases and antiproteases. We report here the cloning and pattern of expression of cerebroglycan, a glycosylphosphatidylinositol (GPI)-anchored HSPG that is found in the developing rat brain (previously referred to as HSPG M13; Herndon, M. E., and A. D. Lander. 1990. Neuron. 4:949-961). The cerebroglycan core protein has a predicted molecular mass of 58.6 kD and five potential heparan sulfate attachment sites. Together with glypican (David, G., V. Lories, B. Decock, P. Marynen, J.-J. Cassiman, and H. Van den Berghe. 1990. J. Cell Biol. 111:3165-3176), it defines a family of integral membrane HSPGs characterized by GPI linkage and conserved structural motifs, including a pattern of 14 cysteine residues that is absolutely conserved. Unlike other known integral membrane HSPGs, including glypican and members of the syndecan family of transmembrane proteoglycans, cerebroglycan is expressed in only one tissue: the nervous system. In situ hybridization experiments at several developmental stages strongly suggest that cerebroglycan message is widely and transiently expressed by immature neurons, appearing around the time of final mitosis and disappearing after cell migration and axon outgrowth have been completed. These results suggest that cerebroglycan may fulfill a function related to the motile behaviors of developing neurons.

Cell Differentiation

Gene Expression

DNA, Complementary - genetics

Molecular Sequence Data

Neurons - cytology

DNA Primers - chemistry

Heparitin Sulfate - metabolism

Restriction Mapping

Glypicans

In Situ Hybridization

Nervous System - growth & development

Base Sequence

Cloning, Molecular

Membrane Proteins - metabolism

Neurons - metabolism

Amino Acid Sequence

Membrane Proteins - genetics

RNA, Messenger - genetics

Proteoglycans - metabolism

Glycosylphosphatidylinositols

Peptide Fragments - chemistry

Animals

Membrane Proteins - chemistry

Heparan Sulfate Proteoglycans

Mice

Proteoglycans - genetics

Details

Title: Subtitle: Cerebroglycan: an integral membrane heparan sulfate proteoglycan that is unique to the developing nervous system and expressed specifically during neuronal differentiation
Creators: Christopher S Stipp - Department of Biology, Massachusetts Institute of Technology, Cambridge 02139
E David Litwack
Arthur D Lander
Resource Type: Journal article
Publication Details: The Journal of cell biology, Vol.124(1-2), pp.149-160
Publisher: United States
DOI: 10.1083/jcb.124.1.149
PMID: 8294498
ISSN: 0021-9525
eISSN: 1540-8140
Grant note: NS26862 / NINDS NIH HHS
Language: English
Date published: 01/1994
Academic Unit: Molecular Physiology and Biophysics; Biology
Record Identifier: 9983991983502771

Metrics

22 Record Views

158 Times Cited - Web of Science

See more details