Journal article
Changes in metabolic landscapes shape divergent but distinct mutational signatures and cytotoxic consequences of redox stress
Nucleic acids research, Vol.51(10), pp.5056-5072
06/09/2023
DOI: 10.1093/nar/gkad305
PMCID: PMC10250236
PMID: 37078607
Abstract
Mutational signatures discerned in cancer genomes, in aging tissues and in cells exposed to toxic agents, reflect complex processes underlying transformation of cells from normal to dysfunctional. Due to its ubiquitous and chronic nature, redox stress contributions to cellular makeover remain equivocal. The deciphering of a new mutational signature of an environmentally-relevant oxidizing agent, potassium bromate, in yeast single strand DNA uncovered a surprising heterogeneity in the mutational signatures of oxidizing agents. NMR-based analysis of molecular outcomes of redox stress revealed profound dissimilarities in metabolic landscapes following exposure to hydrogen peroxide versus potassium bromate. The predominance of G to T substitutions in the mutational spectra distinguished potassium bromate from hydrogen peroxide and paraquat and mirrored the observed metabolic changes. We attributed these changes to the generation of uncommon oxidizing species in a reaction with thiol-containing antioxidants; a nearly total depletion of intracellular glutathione and a paradoxical augmentation of potassium bromate mutagenicity and toxicity by antioxidants. Our study provides the framework for understanding multidimensional processes triggered by agents collectively known as oxidants. Detection of increased mutational loads associated with potassium bromate-related mutational motifs in human tumors may be clinically relevant as a biomarker of this distinct type of redox stress.
Details
- Title: Subtitle
- Changes in metabolic landscapes shape divergent but distinct mutational signatures and cytotoxic consequences of redox stress
- Creators
- Natalya P Degtyareva - National Institutes of HealthVictoria C Placentra - National Institutes of HealthScott A Gabel - National Institutes of HealthLeszek J Klimczak - National Institutes of HealthDmitry A Gordenin - National Institutes of HealthBrett A Wagner - University of IowaGarry R Buettner - University of IowaGeoffrey A Mueller - National Institutes of HealthTatyana I Smirnova - North Carolina State UniversityPaul W Doetsch - National Institutes of Health
- Resource Type
- Journal article
- Publication Details
- Nucleic acids research, Vol.51(10), pp.5056-5072
- DOI
- 10.1093/nar/gkad305
- PMID
- 37078607
- PMCID
- PMC10250236
- ISSN
- 0305-1048
- eISSN
- 1362-4962
- Grant note
- Z1AES103328 / NIH HHS
- Language
- English
- Electronic publication date
- 04/20/2023
- Date published
- 06/09/2023
- Academic Unit
- Radiation Oncology
- Record Identifier
- 9984398346302771
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