Journal article
Changes in the cell surface protein composition of human alveolar macrophages induced by smoking
Cancer research (Chicago, Ill.), Vol.47(12), pp.3072-3082
06/15/1987
PMID: 3555768
Abstract
The cell surface proteins of human alveolar macrophages obtained from nonsmokers have been compared to those of alveolar macrophages obtained from smokers. Proteins of nonsmokers' alveolar macrophages surface labeled with 125I differed from those of smokers' alveolar macrophages, as shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Three major radiolabeled proteins with molecular weights of 183,000, 80,000, and 30,000 were identified in fresh smokers' cells. The major radiolabeled protein of nonsmokers' macrophages had an apparent molecular weight of approximately 183,000. Affinity chromatography suggested the Mr 183,000 protein is a mannose receptor. In contrast, the molecular weight of the major radiolabeled protein of smokers' alveolar macrophages was approximately 30,000; the Mr 183,000 protein was less prominent. When nonsmokers' alveolar macrophages were cultured in vitro before 125I labeling, the cell surface protein pattern changed to resemble that of smokers' alveolar macrophages; the Mr 183,000 protein could no longer be detected on the cell surface, whereas a Mr 80,000 protein was increased in quantity and a new Mr 30,000 protein was detected. Nonadherent macrophages showed similar changes in their surface-labeled proteins but also contained a new prominently labeled Mr 70,000 protein. Limited proteolysis peptide mapping with five different enzymes did not reveal any evidence of homology among the Mr 183,000, 80,000, 70,000, and 30,000 proteins. The differences in cell surface protein composition between alveolar macrophages of smokers and nonsmokers may reflect their functional capabilities or their state of "activation" and may be mechanistically important in the development of various pulmonary diseases seen in smokers including cancer. These results also demonstrate that major changes in the surface proteins of the human alveolar macrophage plasma membrane can occur rapidly following manipulation.
Details
- Title: Subtitle
- Changes in the cell surface protein composition of human alveolar macrophages induced by smoking
- Creators
- K M SkubitzD W NorthfeltS E McGowanJ R Hoidal
- Resource Type
- Journal article
- Publication Details
- Cancer research (Chicago, Ill.), Vol.47(12), pp.3072-3082
- Publisher
- United States
- PMID
- 3555768
- ISSN
- 0008-5472
- eISSN
- 1538-7445
- Grant note
- R01-CA-36248 / NCI NIH HHS KO4-HL-32329 / NHLBI NIH HHS R01-HL-01308 / NHLBI NIH HHS
- Language
- English
- Date published
- 06/15/1987
- Academic Unit
- International Programs; Internal Medicine
- Record Identifier
- 9983983667602771
Metrics
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