Journal article
Characteristics of Monoclonal Antibodies to the Lactate Dehydrogenase-Elevating Virus
Intervirology, Vol.27(1), pp.53-60
1987
DOI: 10.1159/000149715
PMID: 3610572
Abstract
Spleen cells, from BALB/c mice that had been infected with lactate dehydrogenase-elevating virus (LDV) for 6 days and that had or had not been previously immunized with glutaraldehyde-inactivated LDV, were fused with NS-1 myeloma cells. The fusion frequency was at least 10 times higher than with spleen cells of normal or chronically infected mice. Only 1 of 297 wells containing hybridomas prepared with spleens from unprimed 6-day-infected mice produced LDV-specific monoclonal antibodies (mAb). In contrast, when mice were immunized with inactivated LDV before infection, 33 of 73 hybridoma-containing wells screened were LDV-specific. The mAbs produced were mainly of IgG2a, IgM, and IgG, subclasses and exhibited an identical characteristic staining pattern of LDV-infected cultured macrophages. A single mAb of IgG2b isotype yielded a different staining pattern. Western blotting showed that all of 12 mAbs analyzed in more detail were specific for the LDV glycoprotein, VP-3, but none of these neutralized the infectivity of the homologous strain of LDV. They also did not significantly protect immunosuppressed 10-month-old C58 mice against LDV-induced paralytic poliomyelitis, as does passive immunization with polyclonal mouse anti-LDV IgG.
Details
- Title: Subtitle
- Characteristics of Monoclonal Antibodies to the Lactate Dehydrogenase-Elevating Virus
- Creators
- John T HartyStephen P.K ChanPeter G.W Plagemann
- Resource Type
- Journal article
- Publication Details
- Intervirology, Vol.27(1), pp.53-60
- Publisher
- Basel, Switzerland
- DOI
- 10.1159/000149715
- PMID
- 3610572
- ISSN
- 0300-5526
- eISSN
- 1423-0100
- Number of pages
- 8
- Language
- English
- Date published
- 1987
- Academic Unit
- Pathology
- Record Identifier
- 9984047682802771
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