Characterization and expression analysis of Staphylococcus aureus pathogenicity island 3. Implications for the evolution of staphylococcal pathogenicity islands

Jeremy M Yarwood; John K McCormick; Michael L Paustian; Paul M Orwin; Vivek Kapur; Patrick M Schlievert

doi:10.1074/jbc.M111661200

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Characterization and expression analysis of Staphylococcus aureus pathogenicity island 3. Implications for the evolution of staphylococcal pathogenicity islands

Journal article

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Peer reviewed

Characterization and expression analysis of Staphylococcus aureus pathogenicity island 3. Implications for the evolution of staphylococcal pathogenicity islands

Jeremy M Yarwood, John K McCormick, Michael L Paustian, Paul M Orwin, Vivek Kapur and Patrick M Schlievert

The Journal of biological chemistry, Vol.277(15), pp.13138-13147

04/12/2002

DOI: 10.1074/jbc.M111661200

PMID: 11821418

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Abstract

We describe the complete sequence of the 15.9-kb staphylococcal pathogenicity island 3 encoding staphylococcal enterotoxin serotypes B, K, and Q. The island, which meets the generally accepted definition of pathogenicity islands, contains 24 open reading frames potentially encoding proteins of more than 50 amino acids, including an apparently functional integrase. The element is bordered by two 17-bp direct repeats identical to those found flanking staphylococcal pathogenicity island 1. The island has extensive regions of homology to previously described pathogenicity islands, particularly staphylococcal pathogenicity islands 1 and bov. The expression of 22 of the 24 open reading frames contained on staphylococcal pathogenicity island 3 was detected either in vitro during growth in a laboratory medium or serum or in vivo in a rabbit model of toxic shock syndrome using DNA microarrays. The effect of oxygen tension on staphylococcal pathogenicity island 3 gene expression was also examined. By comparison with the known staphylococcal pathogenicity islands in the context of gene expression described here, we propose a model of pathogenicity island origin and evolution involving specialized transduction events and addition, deletion, or recombination of pathogenicity island "modules."

Staphylococcus aureus - genetics

Genes, Bacterial

Rabbits

Animals

Base Sequence

Molecular Sequence Data

Staphylococcus aureus - pathogenicity

DNA, Bacterial

Evolution, Molecular

Details

Title: Subtitle: Characterization and expression analysis of Staphylococcus aureus pathogenicity island 3. Implications for the evolution of staphylococcal pathogenicity islands
Creators: Jeremy M Yarwood - Department of Microbiology, University of Minnesota Medical School, 420 Delaware Street SE, Minneapolis, MN 55455, USA
John K McCormick
Michael L Paustian
Paul M Orwin
Vivek Kapur
Patrick M Schlievert
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.277(15), pp.13138-13147
DOI: 10.1074/jbc.M111661200
PMID: 11821418
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: United States
Grant note: AI 22159 / NIAID NIH HHS
Language: English
Date published: 04/12/2002
Academic Unit: Microbiology and Immunology; Internal Medicine
Record Identifier: 9984001216102771

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