Characterization of (4-hydroxy-3-nitrophenyl)acetyl (NP)-specific germinal center B cells and antigen-binding B220- cells after primary NP challenge in mice

Kristy L Wolniak; Randolph J Noelle; Thomas J Waldschmidt

doi:10.4049/jimmunol.177.4.2072

Back

Characterization of (4-hydroxy-3-nitrophenyl)acetyl (NP)-specific germinal center B cells and antigen-binding B220- cells after primary NP challenge in mice

Journal article

Peer reviewed

Characterization of (4-hydroxy-3-nitrophenyl)acetyl (NP)-specific germinal center B cells and antigen-binding B220- cells after primary NP challenge in mice

Kristy L Wolniak, Randolph J Noelle and Thomas J Waldschmidt

The Journal of immunology (1950), Vol.177(4), pp.2072-2079

08/15/2006

DOI: 10.4049/jimmunol.177.4.2072

PMID: 16887965

View Online

Abstract

Previous studies examining the primary germinal center (GC) response to SRBC in mice demonstrated a steady ratio of IgM(+) to isotype-switched GC B cells and a persistent population of GC B cells with a founder phenotype. These characteristics held true at the inductive, plateau, and dissociative phases of the GC response, suggesting a steady-state environment. To test whether these characteristics apply to the primary response of other T cell-dependent Ags, the present study examined the GC response after challenge with (4-hydroxy-3-nitrophenyl)acetyl (NP) in C57BL/6 mice. Multiparameter flow cytometric analysis was used to assess the phenotype of splenic NP-reactive cells at multiple time points after immunization. Results of these studies demonstrated the characteristics of the SRBC-induced GC reaction to be fully maintained in the NP response. In particular, there was a steady ratio of nonswitched to switched B cells, with the majority of NP-reactive GC B cells displaying IgM. In addition, a substantial frequency of B220(-) NP-binding cells was observed in the spleen at later time points after NP challenge. Although these cells were IgE(+), they were found to express both kappa and lambda L chains and display the high-affinity IgE Fc (FcepsilonRI) receptor, suggesting that this population is not of B cell origin. Adoptive transfer studies further demonstrated the B220(-) NP-binding subset to be derived from the myeloid lineage.

Haptens - administration & dosage

Leukocyte Common Antigens - metabolism

Germinal Center - immunology

Mice, Inbred C57BL

Epitopes, B-Lymphocyte - administration & dosage

Nitrophenols - administration & dosage

Epitopes, B-Lymphocyte - immunology

Protein Binding - immunology

Haptens - immunology

Mice, Knockout

Nitrophenols - immunology

Animals

Germinal Center - metabolism

B-Lymphocyte Subsets - metabolism

Phenylacetates

B-Lymphocyte Subsets - immunology

Female

Mice

Germinal Center - cytology

Details

Title: Subtitle: Characterization of (4-hydroxy-3-nitrophenyl)acetyl (NP)-specific germinal center B cells and antigen-binding B220- cells after primary NP challenge in mice
Creators: Kristy L Wolniak - Department of Pathology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
Randolph J Noelle
Thomas J Waldschmidt
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.177(4), pp.2072-2079
DOI: 10.4049/jimmunol.177.4.2072
PMID: 16887965
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Publisher: United States
Grant note: T32 GM007337 / NIGMS NIH HHS R37 AI 26296 / NIAID NIH HHS R01 AA 014400 / NIAAA NIH HHS
Language: English
Date published: 08/15/2006
Academic Unit: Pathology
Record Identifier: 9984047660002771

Metrics

21 Record Views

35 Times Cited - Web of Science