Characterization of 5'-heterogeneity of the rat GLUT4/muscle-adipose glucose transporter gene product

Ann Louise Olson; Nicholas P Edgington; W Scott Moye-Rowley; Jeffrey E Pessin

doi:10.1210/endo.136.5.7720644

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Characterization of 5'-heterogeneity of the rat GLUT4/muscle-adipose glucose transporter gene product

Journal article

Peer reviewed

Characterization of 5'-heterogeneity of the rat GLUT4/muscle-adipose glucose transporter gene product

Ann Louise Olson, Nicholas P Edgington, W Scott Moye-Rowley and Jeffrey E Pessin

Endocrinology (Philadelphia), Vol.136(5), pp.1962-1968

05/1995

DOI: 10.1210/endo.136.5.7720644

PMID: 7720644

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Abstract

To examine the mechanisms responsible for tissue-specific, nutritional, and metabolic regulation of the GLUT4/muscle-adipose specific glucose transporter, we isolated and characterized the properties of the rat GLUT4 gene. Examination of the sequenced 2.5-kilobase flanking DNA revealed substantial identity with that of the mouse and human GLUT4 genes, with the greatest degree of sequence identity within the proximal 1000 basepairs up-stream of the GLUT4 open reading frame. Primer extension analysis identified a unique single transcription initiation site 176 basepairs up-stream from the start of translation. However, ribonuclease mapping revealed the presence of a previously undescribed alternatively spliced form of GLUT4 messenger RNA. Approximately 75% of the GLUT4 transcripts consisted of a fully spliced messenger RNA, and 25% was expressed as an unspliced intron-containing species. The ratios of 5' spliced and unspliced messages were invariant in adipose, cardiac, and skeletal muscle tissues. In vitro translation of reporter constructs containing both the spliced and unspliced leader demonstrated a functional difference between these two transcripts, with the unspliced form translated approximately 5-fold more than the fully spliced species. These data demonstrate the presence of 5'-heterogeneity of the GLUT4 transcripts, which underlies differences in translational efficiency in vitro.

Adipose Tissue - metabolism

Alternative Splicing

Amino Acid Sequence

Animals

Base Sequence

DNA Primers

Exons

Genomic Library

Glucose Transporter Type 4

Introns

Molecular Sequence Data

Monosaccharide Transport Proteins - biosynthesis

Monosaccharide Transport Proteins - genetics

Muscle Proteins

Muscles - metabolism

Open Reading Frames

Protein Biosynthesis

Rats - genetics

Restriction Mapping

RNA, Messenger - analysis

RNA, Messenger - biosynthesis

Sequence Homology, Nucleic Acid

Transcription, Genetic

Details

Title: Subtitle: Characterization of 5'-heterogeneity of the rat GLUT4/muscle-adipose glucose transporter gene product
Creators: Ann Louise Olson - University of Iowa
Nicholas P Edgington - University of Iowa
W Scott Moye-Rowley - University of Iowa
Jeffrey E Pessin - University of Iowa
Resource Type: Journal article
Publication Details: Endocrinology (Philadelphia), Vol.136(5), pp.1962-1968
DOI: 10.1210/endo.136.5.7720644
PMID: 7720644
ISSN: 0013-7227
eISSN: 1945-7170
Grant note: DK-42452 / NIDDK NIH HHS DK-25295 / NIDDK NIH HHS DK-44612 / NIDDK NIH HHS
Language: English
Date published: 05/1995
Academic Unit: Molecular Physiology and Biophysics; Internal Medicine
Record Identifier: 9984297615202771

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11 Times Cited - Web of Science