Journal article
Characterization of Campylobacter jejuni–Neutrophil Interactions
Current protocols, Vol.1(11), pp.e294-n/a
11/2021
DOI: 10.1002/cpz1.294
Abstract
Campylobacter jejuni is the leading cause of bacterial‐derived gastroenteritis worldwide, infecting 96 million individuals annually. During infection, inflammation and tissue pathology occur in the lower gastrointestinal tract, including the recruitment of leukocytes. Neutrophils are the most abundant leukocyte in humans, and recruitment is associated with bacterial infections and the development of various inflammatory disorders, including inflammatory bowel disease. Neutrophils possess three main antibacterial functions: phagocytosis and degradation of microbes, degranulation to release antimicrobial proteins, and extrusion of neutrophil extracellular traps (NETs). Because neutrophils are recruited to the site of C. jejuni infection and they are associated with damaging inflammation in other diseases, it is imperative to understand the immunopathology that occurs during C. jejuni infection and thoroughly study the neutrophil response to the pathogen. Detailed protocols for human and ferret neutrophil isolations, neutrophil gentamicin protection assay, neutrophil activation flow cytometry assay, NET induction and quantification, and neutrophil western blot analysis are included in this article. © 2021 Wiley Periodicals LLC.
Basic Protocol 1: Isolation of human and ferret neutrophils
Basic Protocol 2: Neutrophil gentamicin protection assay
Basic Protocol 3: Neutrophil activation flow cytometry analyses
Basic Protocol 4: Neutrophil extracellular trap induction and quantification
Basic Protocol 5: Western blot detection of neutrophil‐derived antimicrobial proteins
Details
- Title: Subtitle
- Characterization of Campylobacter jejuni–Neutrophil Interactions
- Creators
- Sean M. Callahan - University of Tennessee at KnoxvilleTrevor J. Hancock - University of Tennessee at KnoxvilleJeremiah G. Johnson - University of Tennessee at Knoxville
- Resource Type
- Journal article
- Publication Details
- Current protocols, Vol.1(11), pp.e294-n/a
- DOI
- 10.1002/cpz1.294
- ISSN
- 2691-1299
- eISSN
- 2691-1299
- Number of pages
- 19
- Grant note
- University of Tennessee
- Language
- English
- Date published
- 11/2021
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984696575702771
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