Characterization of Extracellular Vesicles from Entamoeba histolytica Identifies Roles in Intercellular Communication That Regulates Parasite Growth and Development

Manu Sharma; Pedro Morgado; Hanbang Zhang; Gretchen Ehrenkaufer; Dipak Manna; Upinder Singh

doi:10.1128/IAI.00349-20

Back

Characterization of Extracellular Vesicles from Entamoeba histolytica Identifies Roles in Intercellular Communication That Regulates Parasite Growth and Development

Journal article

Peer reviewed

Characterization of Extracellular Vesicles from Entamoeba histolytica Identifies Roles in Intercellular Communication That Regulates Parasite Growth and Development

Manu Sharma, Pedro Morgado, Hanbang Zhang, Gretchen Ehrenkaufer, Dipak Manna and Upinder Singh

Infection and immunity, Vol.88(10), e00349

09/18/2020

DOI: 10.1128/IAI.00349-20

PMID: 32719158

View Online

Abstract

Extracellular vesicles (EVs) secreted by eukaryotic and prokaryotic cells to transport lipids, proteins, and nucleic acids to the external environment have important roles in cell-cell communication through cargo transfer. We identified and characterized EVs from , a protozoan parasite and a human pathogen. Conditioned medium from amebic parasites contained particles consistent with the expected size and morphology of EVs. Mass spectrometry was used to characterize the EV proteome and showed that it was enriched in common exosome marker proteins, including proteins associated with vesicle formation, cell signaling, and metabolism, as well as cytoskeletal proteins. Additionally, the EVs were found to selectively package small RNAs (sRNA), which were protected within the vesicles against RNase treatment. Sequencing analysis of the sRNA contained in EVs revealed that the majority were 27 nucleotides (nt) in size and represented a subset of the cellular antisense small RNA population that has previously been characterized in RNA interference (RNAi) pathway proteins, including Argonaute, were also present in amebic EVs. Interestingly, we found that the amebic EVs impacted intercellular communication between parasites and altered encystation efficiency. EVs isolated from encysting parasites promoted encystation in other parasites, whereas EVs from metabolically active trophozoites impeded encystation. Overall, the data reveal that secrete EVs that are similar in size and shape to previously characterized exosomes from other organisms and that these EVs contain a defined protein and small RNA cargo and have roles in intercellular communication among parasites and influence growth kinetics.

Biomarkers - metabolism

Cell Communication

Entamoeba histolytica - cytology

Entamoeba histolytica - growth & development

Entamoeba histolytica - metabolism

Exosomes - metabolism

Extracellular Vesicles - metabolism

Life Cycle Stages

Parasite Encystment

Proteome

Protozoan Proteins - metabolism

RNA, Protozoan - genetics

RNA, Protozoan - metabolism

RNA, Small Untranslated - genetics

RNA, Small Untranslated - metabolism

Details

Title: Subtitle: Characterization of Extracellular Vesicles from Entamoeba histolytica Identifies Roles in Intercellular Communication That Regulates Parasite Growth and Development
Creators: Manu Sharma - Stanford Medicine
Pedro Morgado - Stanford Medicine
Hanbang Zhang - Stanford Medicine
Gretchen Ehrenkaufer - Stanford Medicine
Dipak Manna - Stanford Medicine
Upinder Singh - Stanford Medicine
Resource Type: Journal article
Publication Details: Infection and immunity, Vol.88(10), e00349
DOI: 10.1128/IAI.00349-20
PMID: 32719158
ISSN: 0019-9567
eISSN: 1098-5522
Grant note: R21 AI125764 / NIAID NIH HHS P30 CA124435 / NCI NIH HHS R01 AI121084 / NIAID NIH HHS
Language: English
Date published: 09/18/2020
Academic Unit: Internal Medicine
Record Identifier: 9985244664002771

Metrics

1 Record Views

48 Times Cited - Web of Science