Characterization of Immunophenotypic Aberrancies in 200 Cases of B Acute Lymphoblastic Leukemia

Adam C SEEGMILLER; Steven H KROFT; Nitin J KARANDIKAR; Robert W MCKENNA

doi:10.1309/AJCP8G5RMTWUEMUU

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Characterization of Immunophenotypic Aberrancies in 200 Cases of B Acute Lymphoblastic Leukemia

Journal article

Open access

Peer reviewed

Characterization of Immunophenotypic Aberrancies in 200 Cases of B Acute Lymphoblastic Leukemia

Adam C SEEGMILLER, Steven H KROFT, Nitin J KARANDIKAR and Robert W MCKENNA

American journal of clinical pathology, Vol.132(6), pp.940-949

2009

DOI: 10.1309/AJCP8G5RMTWUEMUU

PMID: 19926587

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Abstract

Morphologic distinction of leukemic lymphoblasts in B acute lymphoblastic leukemia (B-ALL) from their nonneoplastic counterparts in bone marrow (hematogones) can be difficult. Thus, the presence of aberrant antigen expression detectable by flow cytometry may be critical for diagnosis of B-ALL and detection of minimal residual disease. The current study examined the immunophenotype of B-lineage leukemic lymphoblasts in 200 consecutive, unique, pretreatment patient specimens. We found that all cases of B-ALL exhibited multiple immunophenotypic aberrancies by which they can be distinguished from hematogones. The most frequent aberrancies were uniform or a spectrum of expression of terminal deoxynucleotidyl transferase and CD34, underexpression of CD45, overexpression of CD10, underexpression of CD38, and underexpression of CD20. Asynchronous coexpression of CD34 and CD20 was also frequently observed. Of the 200 cases, 86.5% expressed myeloid-associated antigens, and 19.0% expressed 3 or more. Of 200 cases, 9.0% aberrantly expressed T cell-associated antigens. There were significant differences in antigen-expression patterns between adult and pediatric B-ALL. Specific aberrancies correlate with recurrent cytogenetic abnormalities in B-ALL.

Hematologic and hematopoietic diseases

Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis

Biological and medical sciences

Investigative techniques, diagnostic techniques (general aspects)

Medical sciences

Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques

Details

Title: Subtitle: Characterization of Immunophenotypic Aberrancies in 200 Cases of B Acute Lymphoblastic Leukemia
Creators: Adam C SEEGMILLER - Department of Pathology, University of Texas Southwestern Medical Center, Dallas, United States
Steven H KROFT - Department of Pathology, Medical College of Wisconsin, Milwaukee, United States
Nitin J KARANDIKAR - Department of Pathology, University of Texas Southwestern Medical Center, Dallas, United States
Robert W MCKENNA - Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis, United States
Resource Type: Journal article
Publication Details: American journal of clinical pathology, Vol.132(6), pp.940-949
DOI: 10.1309/AJCP8G5RMTWUEMUU
PMID: 19926587
NLM abbreviation: Am J Clin Pathol
ISSN: 0002-9173
eISSN: 1943-7722
Publisher: American Society of Clinical Pathologists; Chicago, IL
Language: English
Date published: 2009
Academic Unit: Pathology
Record Identifier: 9984047628102771

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