Journal article
Characterization of Lef-1 Promoter Segments that Facilitate Inductive Developmental Expression in Skin
Journal of investigative dermatology, Vol.123(2), pp.264-274
08/2004
DOI: 10.1111/j.0022-202X.2004.23201.x
PMCID: PMC1803077
PMID: 15245424
Abstract
Lymphoid Enhancer Factor 1 (Lef-1) is an important developmental transcription factor required for the inductive formation of several epithelial-derived organs including hair follicles. Inductive expression of Lef-1 mRNA is tightly regulated during embryo development, suggesting the involvement of a highly regulated promoter. In vitro analysis of the Lef-1 gene has demonstrated the existence of at least two spatially distinct promoters with multiple transcriptional start sites that are responsive to the canonical Wnt/β-catenin pathway. Regions of the Lef-1 promoter required for inductive regulation in vivo, however, have yet to be determined. To this end, we utilized LacZ-reporter transgenic mice to define segments of the human Lef-1 promoter capable of reproducing mesenchymal- or epithelial-restricted transcriptional patterns of Lef-1 expression during hair and vibrissa follicle development. These studies have revealed that a 110 bp Wnt/β-catenin-responsive element, contained within a minimal 2.5 kb Lef-1 promoter, plays an important role in regulating mesenchymal, and potentially epithelial, expression during follicle development in mouse embryos. This 2.5 kb Lef-1 promoter also demonstrated inductive mesenchymal expression during postnatal anagen stage hair-follicle cycling. Additionally, analysis of Lef-1 promoter expression revealed previously uncharacterized regions of endogenous Lef-1 expression seen in the sebaceous glands of vibrissa and hair follicles in transgenic lines harboring the minimal Lef-1 promoter and additional intronic sequences. In summary, these studies have begun to dissect the transcriptional diversity of the human Lef-1 promoter during the hair/vibrissa follicle and sebaceous gland formation.
Details
- Title: Subtitle
- Characterization of Lef-1 Promoter Segments that Facilitate Inductive Developmental Expression in Skin
- Creators
- Xiaoming Liu - Department of Anatomy and Cell Biology, University of Iowa College of Medicine, Iowa City, Iowa, USARyan R Driskell - Department of Anatomy and Cell Biology, University of Iowa College of Medicine, Iowa City, Iowa, USAMeihui Luo - Department of Anatomy and Cell Biology, University of Iowa College of Medicine, Iowa City, Iowa, USADuane Abbott - Department of Anatomy and Cell Biology, University of Iowa College of Medicine, Iowa City, Iowa, USAMohammed Filali - Department of Anatomy and Cell Biology, University of Iowa College of Medicine, Iowa City, Iowa, USANingli Cheng - Department of Anatomy and Cell Biology, University of Iowa College of Medicine, Iowa City, Iowa, USACurt D Sigmund - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, Iowa, USAJohn F Engelhardt - Department of Anatomy and Cell Biology, University of Iowa College of Medicine, Iowa City, Iowa, USA
- Resource Type
- Journal article
- Publication Details
- Journal of investigative dermatology, Vol.123(2), pp.264-274
- Publisher
- Elsevier Inc
- DOI
- 10.1111/j.0022-202X.2004.23201.x
- PMID
- 15245424
- PMCID
- PMC1803077
- ISSN
- 0022-202X
- eISSN
- 1523-1747
- Language
- English
- Date published
- 08/2004
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Molecular Physiology and Biophysics; Anatomy and Cell Biology; Radiation Oncology; Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984025458502771
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