Journal article
Characterization of Neutrophil Function in Human Cutaneous Leishmaniasis Caused by Leishmania braziliensis
PLoS neglected tropical diseases, Vol.10(5), pp.e0004715-e0004715
05/2016
DOI: 10.1371/journal.pntd.0004715
PMCID: PMC4864077
PMID: 27167379
Abstract
Infection with different Leishmania spp. protozoa can lead to a variety of clinical syndromes associated in many cases with inflammatory responses in the skin. Although macrophages harbor the majority of parasites throughout chronic infection, neutrophils are the first inflammatory cells to migrate to the site of infection. Whether neutrophils promote parasite clearance or exacerbate disease in murine models varies depending on the susceptible or resistant status of the host. Based on the hypothesis that neutrophils contribute to a systemic inflammatory state in humans with symptomatic L. braziliensis infection, we evaluated the phenotype of neutrophils from patients with cutaneous leishmaniasis (CL) during the course of L. braziliensis infection. After in vitro infection with L. braziliensis, CL patient neutrophils produced more reactive oxygen species (ROS) and higher levels of CXCL8 and CXCL9, chemokines associated with recruitment of neutrophils and Th1-type cells, than neutrophils from control healthy subjects (HS). Despite this, CL patient and HS neutrophils were equally capable of phagocytosis of L. braziliensis. There was no difference between the degree of activation of neutrophils from CL versus healthy subjects, assessed by CD66b and CD62L expression using flow cytometry. Of interest, these studies revealed that both parasite-infected and bystander neutrophils became activated during incubation with L. braziliensis. The enhanced ROS and chemokine production in neutrophils from CL patients reverted to baseline after treatment of disease. These data suggest that the circulating neutrophils during CL are not necessarily more microbicidal, but they have a more pro-inflammatory profile after parasite restimulation than neutrophils from healthy subjects.
Details
- Title: Subtitle
- Characterization of Neutrophil Function in Human Cutaneous Leishmaniasis Caused by Leishmania braziliensis
- Creators
- Jacilara Conceição - Serviço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia, Salvador, Bahia, BrazilRichard Davis - Interdisciplinary Program in Immunology, University of Iowa, Iowa City, Iowa, United States of AmericaPedro Paulo Carneiro - Serviço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia, Salvador, Bahia, BrazilAngela Giudice - Serviço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia, Salvador, Bahia, BrazilAline C Muniz - Serviço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia, Salvador, Bahia, BrazilMary E Wilson - Department of Veterans' Affairs Medical Center, Iowa City, Iowa, United States of AmericaEdgar M Carvalho - Centro de Pesquisa Gonçalo Moniz, FIOCRUZ - BA, Salvador, Bahia, BrazilOlívia Bacellar - Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais - INCT-DT (CNPq/MCT), Salvador, Bahia, Brazil
- Resource Type
- Journal article
- Publication Details
- PLoS neglected tropical diseases, Vol.10(5), pp.e0004715-e0004715
- DOI
- 10.1371/journal.pntd.0004715
- PMID
- 27167379
- PMCID
- PMC4864077
- NLM abbreviation
- PLoS Negl Trop Dis
- ISSN
- 1935-2727
- eISSN
- 1935-2735
- Publisher
- United States
- Grant note
- T32 AI007511 / NIAID NIH HHS R01 AI076233 / NIAID NIH HHS I01 BX001983 / BLRD VA P50 AI030639 / NIAID NIH HHS
- Language
- English
- Date published
- 05/2016
- Academic Unit
- Microbiology and Immunology; International Programs; Epidemiology; Internal Medicine
- Record Identifier
- 9984001202902771
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