Journal article
Characterization of Small RNAs and Their Targets from Fusarium oxysporum Infected and Noninfected Cotton Root Tissues
Plant molecular biology reporter, Vol.34(3), pp.698-706
06/01/2016
DOI: 10.1007/s11105-015-0945-z
Abstract
Genes for host-plant resistant do exist in cotton (Gossypium spp.) but improvement of cotton cultivars with resistance is difficult due to intensive breeding. Identifying molecular-genetic mechanisms associated with disease resistance can offer a new way to combat a serious threat such as Fusarium oxysporum f. sp. vasinfectum (FOV). Here, we captured and annotated "top-layer" of abundantly and specifically expressed cotton root small RNA (sRNA) including microRNA (miR) sequences during FOV pathogenesis using size-directed and adenylated linker-based sRNA cloning strategy. A total of 4116 candidate sRNA sequences with 16 to 30 nucleotide (nt) length were identified from four complementary DNA (cDNA) libraries of noninfected and FOV race 3-infected roots of susceptible ("11970") versus resistant ("Mebane B-1") cotton genotypes (G. hirsutum L.). The highest numbers of sRNA signatures were those with 19-24 nt long in all libraries, and interestingly, the number of sRNAs substantially increased during FOV infection in a resistant genotype, while it sharply decreased in a susceptible genotype. In BLAST analysis, more than 73 % of sRNAs matched Gossypium (G. arboretum L., G. hirsutum, and G. barbadense L.) ESTs. A small percentage of sRNAs matched A. thaliana (1.68 %), T. cacao (1.26 %), fungal (2 %), and other organism (21.33 %) ESTs. mirBase comparisons showed that 4 % of sRNAs were homologous to previously reported plant miRs, among which we predicted novel cotton Ghr-miR-160 that was not registered in the cotton miR database. These major representative sRNA signatures targeted proteins associated with the key biological processes and molecular functions, explaining the molecular mechanisms of the host defense response during the FOV pathogenesis in cotton.
Details
- Title: Subtitle
- Characterization of Small RNAs and Their Targets from Fusarium oxysporum Infected and Noninfected Cotton Root Tissues
- Creators
- U. M. Shapulatov - Ministry of Agriculture and Water ResourcesZ. T. Buriev - Ministry of Agriculture and Water ResourcesM. Ulloa - Plant Stress and Germplasm Development ResearchS. Saha - Crop Science Research LaboratoryE. J. Devor - University of IowaM. S. Ayubov - Ministry of Agriculture and Water ResourcesT. M. Norov - Ministry of Agriculture and Water ResourcesS. E. Shermatov - Ministry of Agriculture and Water ResourcesA. Abdukarimov - Ministry of Agriculture and Water ResourcesJ. N. Jenkins - Crop Science Research LaboratoryI. Y. Abdurakhmonov - Ministry of Agriculture and Water Resources
- Resource Type
- Journal article
- Publication Details
- Plant molecular biology reporter, Vol.34(3), pp.698-706
- Publisher
- Springer Nature
- DOI
- 10.1007/s11105-015-0945-z
- ISSN
- 0735-9640
- eISSN
- 1572-9818
- Number of pages
- 9
- Grant note
- UZB2-31016-TA-09 / Office of International Research Programs (OIRP) of the United States Department of Agriculture (USDA)-Agricultural Research Service (ARS)
- Language
- English
- Date published
- 06/01/2016
- Academic Unit
- Obstetrics and Gynecology
- Record Identifier
- 9984315742602771
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