Journal article
Characterization of Type I Interferon-Associated Chemokines and Cytokines in Lacrimal Glands of Nonobese Diabetic Mice
International journal of molecular sciences, Vol.22(7), p.3767
04/01/2021
DOI: 10.3390/ijms22073767
PMCID: PMC8038628
PMID: 33916486
Abstract
Type I interferons (IFNs) are required for spontaneous lacrimal gland inflammation in the nonobese diabetic (NOD) mouse model of Sjogren's disease, but the consequences of type I IFN signaling are not well-defined. Here, we use RNA sequencing to define cytokine and chemokine genes upregulated in lacrimal glands of NOD mice in a type I IFN-dependent manner. Interleukin (IL)-21 was the highest differentially expressed cytokine gene, and Il21 knockout NOD mice were relatively protected from lacrimal gland inflammation. We defined a set of chemokines upregulated early in disease including Cxcl9 and Cxcl10, which share a receptor, CXCR3. CXCR3(+) T cells were enriched in lacrimal glands with a dominant proportion of CXCR3(+) regulatory T cells. Together these data define the early cytokine and chemokine signals associated with type I IFN-signaling in the development of lacrimal gland inflammation in NOD mice providing insight into the role of type I IFN in autoimmunity development.
Details
- Title: Subtitle
- Characterization of Type I Interferon-Associated Chemokines and Cytokines in Lacrimal Glands of Nonobese Diabetic Mice
- Creators
- Merri-Grace Allred - Roy J. and Lucille A. Carver College of MedicineMichael S. Chimenti - Roy J. and Lucille A. Carver College of MedicineAshley E. Ciecko - Medical College of WisconsinYi-Guang Chen - Medical College of WisconsinScott M. Lieberman - Univ Iowa, Stead Family Dept Pediat, Carver Coll Med, Iowa City, IA 52242 USA
- Resource Type
- Journal article
- Publication Details
- International journal of molecular sciences, Vol.22(7), p.3767
- DOI
- 10.3390/ijms22073767
- PMID
- 33916486
- PMCID
- PMC8038628
- NLM abbreviation
- Int J Mol Sci
- ISSN
- 1661-6596
- eISSN
- 1422-0067
- Publisher
- Mdpi
- Number of pages
- 19
- Grant note
- Carver College of Medicine University of Iowa Vice President for Research EY027731; AI110963 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA University of Iowa Carver College of Medicine Iowa City Veteran's Administration Medical Center Pediatric Rheumatology Research Fund in Memory of Basanta Konar Holden Comprehensive Cancer Center Iowa State Fraternal Order of Eagles
- Language
- English
- Date published
- 04/01/2021
- Academic Unit
- Stead Family Department of Pediatrics; Rheumatology, Allergy, and Immunology; Iowa Institute of Human Genetics
- Record Identifier
- 9984354118002771
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