Journal article
Characterization of a BHK(TK-) cell clone resistant to postattachment entry by herpes simplex virus types 1 and 2
Journal of virology, Vol.71(8), pp.5805-5813
08/1997
DOI: 10.1128/JVI.71.8.5805-5813.1997
PMCID: PMC191835
PMID: 9223469
Abstract
BHK(TK-) cells selected for resistance to polyethylene glycol-mediated fusion give rise to clones that are resistant to herpes simplex virus (HSV) infection. We have characterized one such clone, designated 95-19, and found that it is resistant to entry of HSV type 1 (HSV-1), HSV-2, and the related alphaherpesvirus pseudorabies virus (PRV). Single-step growth experiments show no detectable replication of multiple strains of HSV-1 and HSV-2 on 95-19 cells. Three lines of evidence suggest that these cells are resistant to postattachment entry. (i) Measurements of binding of radiolabeled virus show that heparin-sensitive binding of HSV-1 and HSV-2 to 95-19 cells is identical to binding to BHK(TK-) cells, suggesting that the block to replication occurs after attachment to heparan sulfate proteoglycan. (ii) 95-19 cells exposed to HSV-1 or HSV-2 at high multiplicity show no detectable immediate-early (IE) mRNA expression. (iii) Exposure of attached virus and cells to polyethylene glycol results in partial recovery of both IE gene expression and virus yield in single-step growth. The degrees of recovery of single-step yield and IE gene expression are similar, suggesting that the only block to single-step replication is at the point of virus entry and that these cells are deficient in some cellular factor required for efficient postattachment entry of free virus. 95-19 cells are also highly resistant to entry by cell-to-cell spread, suggesting that the same cellular factor participates in both types of entry.
Details
- Title: Subtitle
- Characterization of a BHK(TK-) cell clone resistant to postattachment entry by herpes simplex virus types 1 and 2
- Creators
- R J Roller - Department of Microbiology, University of Iowa, Iowa City 52242, USAB C Herold - Department of Microbiology, University of Iowa, Iowa City 52242, USA
- Resource Type
- Journal article
- Publication Details
- Journal of virology, Vol.71(8), pp.5805-5813
- DOI
- 10.1128/JVI.71.8.5805-5813.1997
- PMID
- 9223469
- PMCID
- PMC191835
- ISSN
- 0022-538X
- eISSN
- 1098-5514
- Language
- English
- Date published
- 08/1997
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984001121902771
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