Journal article
Characterization of a Staphylococcus aureus Surface Virulence Factor That Promotes Resistance to Oxidative Killing and Infectious Endocarditis
Infection and immunity, Vol.79(1), pp.342-352
01/2011
DOI: 10.1128/IAI.00736-10
PMCID: PMC3019865
PMID: 20937760
Abstract
Staphylococcus aureus
is a prominent human pathogen and a leading cause of community- and hospital-acquired bacterial infections worldwide. Herein, we describe the identification and characterization of the
S. aureus
67.6-kDa hypothetical protein, named for the
s
urface factor promoting resistance to
o
xidative
k
illing (SOK) in this study. Sequence analysis showed that the SOK gene is conserved in all sequenced
S. aureus
strains and homologous to the myosin cross-reactive antigen of
Streptococcus pyogenes.
Immunoblotting and immunofluorescence analysis showed that SOK was copurified with membrane fractions and was exposed on the surface of
S. aureus
Newman and RN4220. Comparative analysis of wild-type
S. aureus
and an isogenic deletion strain indicated that SOK contributes to both resistance to killing by human neutrophils and to oxidative stress. In addition, the
S. aureus sok
deletion strain showed dramatically reduced aortic valve vegetation and bacterial cell number in a rabbit endocarditis model. These results, plus the suspected role of the streptococcal homologue in certain diseases such as acute rheumatic fever, suggest that SOK plays an important role in cardiovascular and other staphylococcal infections.
Details
- Title: Subtitle
- Characterization of a Staphylococcus aureus Surface Virulence Factor That Promotes Resistance to Oxidative Killing and Infectious Endocarditis
- Creators
- Natalia Malachowa - Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455Petra L Kohler - Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455Patrick M Schlievert - Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455Olivia N Chuang - Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455Gary M Dunny - Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455Scott D Kobayashi - Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455Jacek Miedzobrodzki - Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455Gregory A Bohach - Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455Keun Seok Seo - Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.79(1), pp.342-352
- DOI
- 10.1128/IAI.00736-10
- PMID
- 20937760
- PMCID
- PMC3019865
- NLM abbreviation
- Infect Immun
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Publisher
- American Society for Microbiology (ASM)
- Language
- English
- Date published
- 01/2011
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984001146502771
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