Journal article
Characterization of a Trypanosoma cruzi poly(A)-binding protein and its genes
Molecular and biochemical parasitology, Vol.67(2), pp.301-312
1994
DOI: 10.1016/0166-6851(94)00133-2
PMID: 7870134
Abstract
We have characterized the biochemical properties of a 66-kDa poly(A)-binding protein (PABP1) in the protozoan
Trypanosoma cruzi and isolated two classes of cDNAs encoding the protein. In concordance, Southern blots showed the presence of 2 gene copies. The two cDNA classes differ in the length of adenosine-rich segments in the 5′ untranslated region and in point changes scattered throughout the sequence, but their 1650-bp open reading frames encode identical proteins. A single mRNA of 5.5 kb was detected, indicating that the noncoding regions are unusually long. Both the mRNA and the protein are constitutively expressed in all stages of
T. cruzi life cycle. The biochemical properties and sequence comparisons show that the
T. cruzi PABP1 is similar to the PABP1 of other eukaryotic organisms. These results indicate that PABP1 has been conserved throughout eukaryotic evolution.
Details
- Title: Subtitle
- Characterization of a Trypanosoma cruzi poly(A)-binding protein and its genes
- Creators
- João A.N Batista - Departamento de Biologia Celular, Universidade de Brasilia, Brasilia, DF 70910-900, BrazilSantuza M.R Teixeira - University of IowaJohn E Donelson - University of IowaLouis V Kirchhoff - University of IowaCezar Martins de Sá - Universidade de Brasília
- Resource Type
- Journal article
- Publication Details
- Molecular and biochemical parasitology, Vol.67(2), pp.301-312
- Publisher
- Elsevier B.V
- DOI
- 10.1016/0166-6851(94)00133-2
- PMID
- 7870134
- ISSN
- 0166-6851
- eISSN
- 1872-9428
- Language
- English
- Date published
- 1994
- Academic Unit
- Psychiatry; Infectious Diseases; Epidemiology; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984280832602771
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