Journal article
Characterization of a method to study urodynamics and bladder nociception in male and female mice
Lower urinary tract symptoms, Vol.13(2), pp.319-324
04/2021
DOI: 10.1111/luts.12365
PMID: 33202486
Abstract
Objectives Abdominal electromyogram or visceromotor response (VMR) elicited by bladder distension is a validated as a measure of bladder nociception in mice, however it is not without its limitations. The aim of this study is to address some of these limitations and validate voiding evoked VMR as a measure of bladder nociception mice.
Methods Using both male and female C57BL/6 mice we assessed the VMR response to cytometry- induced voiding before and after instillation of 0.5% acetic acid into the bladder. We then delivered intravesical lidocaine to confirm the VMR response as nociceptive. VMR and correlative cystometric bladder pressures were analyzed.
Results We found that the VMR can be evoked by continuous fluid infusion into the bladder of both male and female mice. This response is potentiated after bladder injury and can be attenuated by administration of a local anesthetic, providing strong evidence that this method can be used to evaluate bladder nociception. Further, evaluation of cystometric pressure traces obtained during VMR recording revealed that intercontraction intervals were not altered after bladder injury in either male or female mice. However, we did observe a decrease in peak threshold pressures after bladder injury in female mice, which could be rescued by lidocaine administration.
Conclusions In conclusion, this technique can measure the VMR and bladder nociception associated with voiding in both female and male mice. Although confounds still exist with the use of anesthesia, further exploration of non-anesthetized voiding-evoked VMR is warranted.
Details
- Title: Subtitle
- Characterization of a method to study urodynamics and bladder nociception in male and female mice
- Creators
- Paulome Srivastava - Washington University in St. LouisHenry H. Lai - Washington University in St. LouisAaron D. Mickle - Washington University in St. Louis
- Resource Type
- Journal article
- Publication Details
- Lower urinary tract symptoms, Vol.13(2), pp.319-324
- DOI
- 10.1111/luts.12365
- PMID
- 33202486
- ISSN
- 1757-5664
- eISSN
- 1757-5672
- Publisher
- Wiley
- Number of pages
- 6
- Grant note
- DK115122; DK82315 / National Institute of Diabetes and Digestive and Kidney Diseases; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK)
- Language
- English
- Date published
- 04/2021
- Academic Unit
- Urology
- Record Identifier
- 9985244541302771
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