Journal article
Characterization of a new tissue-engineered human skin equivalent with hair
In vitro cellular & developmental biology. Animal, Vol.35(6), pp.318-326
06/01/1999
DOI: 10.1007/s11626-999-0081-x
PMID: 10476918
Abstract
We designed a new tissue-engineered skin equivalent in which complete pilosebaceous units were integrated. This model was produced exclusively from human fibroblasts and keratinocytes and did not contain any synthetic material. Fibroblasts were cultured for 35 d with ascorbic acid and formed a thick fibrous sheet in the culture dish. The dermal equivalent was composed of stacked fibroblast sheets and exhibited some ultrastructural organization found in normal connective tissues. Keratinocytes seeded on this tissue formed a stratified and cornified epidermis and expressed typical markers of differentiation (keratin 10, filaggrin, and transglutaminase). After 4 wk of culture, a continuous and ultrastructurally organized basement membrane was observed and associated with the expression of laminin and collagen IV and VII. Complete pilosebaceous units were obtained by thermolysin digestion and inserted in this skin equivalent in order to assess the role of the transfollicular route in percutaneous absorption. The presence of hair follicles abolished the lag-time observed during hydrocortisone diffusion and increased significantly its rate of penetration in comparison to the control (skin equivalent with sham hair insertion). Therefore, this new hairy human skin equivalent model allowed an experimental design in which the only variable was the presence of pilosebaceous units and provided new data confirming the importance of hair follicles in percutaneous absorption.
Details
- Title: Subtitle
- Characterization of a new tissue-engineered human skin equivalent with hair
- Creators
- Martine Michel - Université LavalNicolas L'Heureux - Université LavalRoxane Pouliot - Université LavalWen Xu - Université LavalFrançois A Auger - Université LavalLucie Germain - Université Laval
- Resource Type
- Journal article
- Publication Details
- In vitro cellular & developmental biology. Animal, Vol.35(6), pp.318-326
- DOI
- 10.1007/s11626-999-0081-x
- PMID
- 10476918
- NLM abbreviation
- In Vitro Cell Dev Biol Anim
- ISSN
- 1071-2690
- eISSN
- 1543-706X
- Language
- English
- Date published
- 06/01/1999
- Academic Unit
- Anatomy and Cell Biology; Craniofacial Anomalies Research Center
- Record Identifier
- 9984284337602771
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