Characterization of a nontypeable Haemophilus influenzae thermonuclease

Christine Cho; Aroon T Chande; Lokesh Gakhar; Jason Hunt; Margaret R Ketterer; Michael A Apicella

doi:10.1371/journal.pone.0197010

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Characterization of a nontypeable Haemophilus influenzae thermonuclease

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Characterization of a nontypeable Haemophilus influenzae thermonuclease

Christine Cho, Aroon T Chande, Lokesh Gakhar, Jason Hunt, Margaret R Ketterer and Michael A Apicella

PloS one, Vol.13(5), pp.e0197010-e0197010

05/10/2018

DOI: 10.1371/journal.pone.0197010

PMCID: PMC5944969

PMID: 29746527

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Abstract

Nontypeable Haemophilus influenzae (NTHi) has been shown to form biofilms, comprised of extracellular DNA (eDNA), in the middle ear and bronchus during clinical infections. Studies in our laboratory have shown that NTHi possesses a homolog of Staphylococcus aureus thermonuclease (staphylococcal thermonuclease), NTHi nuclease (NTHiNuc, HI_1296). This enzyme had similar size, heat stability, and divalent cation requirements to those of the staphylococcal homolog as determined by light scattering and circular dichroism spectroscopy. Small angle X-ray scattering (SAXS) analysis suggested an overall shape and substrate-binding site comparable to those of staphylococcal nuclease. However, NTH/Nuc was approximately 25-fold more active in fluorescence resonance energy transfer (FRET) activity assay than staphylococcal thermonuclease. Homology modeling implicates shorter NTHiNuc loops near the active site for this enhanced activity.

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Title: Subtitle: Characterization of a nontypeable Haemophilus influenzae thermonuclease
Creators: Christine Cho - University of Iowa
Aroon T Chande - Georgia Institute of Technology
Lokesh Gakhar - University of Iowa
Jason Hunt - Western Illinois University
Margaret R Ketterer - University of Iowa
Michael A Apicella - University of Iowa
Resource Type: Journal article
Publication Details: PloS one, Vol.13(5), pp.e0197010-e0197010
DOI: 10.1371/journal.pone.0197010
PMID: 29746527
PMCID: PMC5944969
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Publisher: PUBLIC LIBRARY SCIENCE
Number of pages: 14
Grant note: AI024616 / National Institute of Allergy and Infectious Diseases; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) T32AI007343 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) P30GM124169 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) R01GM105404 / National Institute of Health project MINOS AI024616 / NIAID; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) Howard Hughes Medical Institute: Medical Research Fellows Program; Howard Hughes Medical Institute P30DK054759 / NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) Howard Hughes Medical Student Fellowship
Language: English
Date published: 05/10/2018
Academic Unit: Microbiology and Immunology; Biochemistry and Molecular Biology; Internal Medicine
Record Identifier: 9984230629702771

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