Characterization of a novel receptor that maps near the natural killer gene complex: demonstration of carbohydrate binding and expression in hematopoietic cells

M J Fernandes; A A Finnegan; L D Siracusa; C Brenner; N N Iscove; B Calabretta

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Characterization of a novel receptor that maps near the natural killer gene complex: demonstration of carbohydrate binding and expression in hematopoietic cells

Journal article

Peer reviewed

Characterization of a novel receptor that maps near the natural killer gene complex: demonstration of carbohydrate binding and expression in hematopoietic cells

M J Fernandes, A A Finnegan, L D Siracusa, C Brenner, N N Iscove and B Calabretta

Cancer research, Vol.59(11), pp.2709-2717

06/01/1999

PMID: 10363996

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Abstract

A novel type II integral membrane protein has been identified in the course of screening for genes overexpressed in a mouse model of chronic myelogenous leukemia blast crisis. This new protein, designated NKCL, consists of a 210-amino acid polypeptide with a short, NH2-terminal cytoplasmic tail of 17 amino acids preceding a transmembrane domain and a COOH-terminal extracellular region. The COOH-terminal 132 amino acids bear typical features of the C-type animal lectin carbohydrate-recognition domain. The Nkcl gene is unique in that it maps just proximal to the region of the genome that encodes group V members of the C-type animal lectin family near the natural killer gene complex on mouse chromosome 6, but its protein product also has features of several group II C-type animal lectins. Most notably, it has a complete Ca2+-binding site 2, which forms part of the sugar-binding site in other members of the family, and binds mannose in a Ca2+-dependent manner. Moreover, its expression is not restricted to natural killer cells, as reported for the majority of group V lectins. Nkcl is expressed in pluripotent myeloid precursors, precursor and mature macrophages, and neutrophils.

Transfection

Amino Acid Sequence

Killer Cells, Natural

Membrane Proteins - genetics

Lectins, C-Type

Leukemia, Myelogenous, Chronic, BCR-ABL Positive - genetics

Molecular Sequence Data

Receptors, Cell Surface - metabolism

Chromosome Mapping

Hematopoietic Stem Cells - metabolism

Neoplasm Proteins - metabolism

Genetic Vectors - genetics

Lectins - metabolism

Blotting, Northern

Animals

Base Sequence

Lectins - genetics

Blast Crisis - genetics

Membrane Proteins - metabolism

Mice

Neoplasm Proteins - genetics

COS Cells

Microscopy, Fluorescence

Receptors, Cell Surface - genetics

Details

Title: Subtitle: Characterization of a novel receptor that maps near the natural killer gene complex: demonstration of carbohydrate binding and expression in hematopoietic cells
Creators: M J Fernandes - Thomas Jefferson University, Department of Microbiology and Immunology, Kimmel Cancer Institute, Philadelphia, Pennsylvania 19107, USA
A A Finnegan
L D Siracusa
C Brenner
N N Iscove
B Calabretta
Resource Type: Journal article
Publication Details: Cancer research, Vol.59(11), pp.2709-2717
Publisher: United States
PMID: 10363996
ISSN: 0008-5472
eISSN: 1538-7445
Grant note: CA46782 / NCI NIH HHS CA21124 / NCI NIH HHS
Language: English
Date published: 06/01/1999
Academic Unit: Biochemistry and Molecular Biology; Internal Medicine
Record Identifier: 9983788599502771

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33 Times Cited - Web of Science