Characterization of a novel staphylococcal enterotoxin-like superantigen, a member of the group V subfamily of pyrogenic toxins

Paul M Orwin; Donald Y M Leung; Timothy J Tripp; Gregory A Bohach; Cathleen A Earhart; Douglas H Ohlendorf; Patrick M Schlievert

doi:10.1021/bi025977q

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Characterization of a novel staphylococcal enterotoxin-like superantigen, a member of the group V subfamily of pyrogenic toxins

Journal article

Peer reviewed

Characterization of a novel staphylococcal enterotoxin-like superantigen, a member of the group V subfamily of pyrogenic toxins

Paul M Orwin, Donald Y M Leung, Timothy J Tripp, Gregory A Bohach, Cathleen A Earhart, Douglas H Ohlendorf and Patrick M Schlievert

Biochemistry (Easton), Vol.41(47), pp.14033-14040

11/26/2002

DOI: 10.1021/bi025977q

PMID: 12437361

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Abstract

Staphylococcus aureus is an important human pathogen, causing a variety of diseases. Major virulence factors of this organism include staphylococcal enterotoxins (SEs) that cause food poisoning and toxic shock syndrome. Our study identified a novel enterotoxin-like protein that is a member of the new subfamily (group V) of pyrogenic toxin superantigens (PTSAgs) and examined its biochemical and immunobiological properties. The gene encoding the SE-like protein is directly 5' of another recently identified PTSAg, SEK. The SE-like protein had a molecular weight of 26000 and an experimentally determined isoelectric point between 7.5 and 8.0. We demonstrated that the PTSAg had many of the biological activities associated with SEs, including superantigenicity, pyrogenicity, and ability to enhance endotoxin shock, but lacked both lethality in rabbits when administered in subcutaneous miniosmotic pumps and emetic activity in monkeys. Recombinant protein stimulated human CD4 and CD8 T cells in a T cell receptor variable region, beta chain (TCRVbeta) specific manner. T cells bearing TCRVbeta 2, 5.1, and 21.3 were significantly stimulated.

Staphylococcus aureus - genetics

Amino Acid Sequence

Humans

Virulence

Pyrogens - chemistry

Pyrogens - isolation & purification

Molecular Sequence Data

Recombinant Proteins - chemistry

Phylogeny

Pyrogens - genetics

DNA Primers

Staphylococcus aureus - pathogenicity

Superantigens - isolation & purification

Bacterial Toxins - chemistry

Bacterial Toxins - isolation & purification

Staphylococcus aureus - chemistry

Superantigens - chemistry

Base Sequence

Superantigens - genetics

Cloning, Molecular

Bacterial Toxins - genetics

Details

Title: Subtitle: Characterization of a novel staphylococcal enterotoxin-like superantigen, a member of the group V subfamily of pyrogenic toxins
Creators: Paul M Orwin - Department of Microbiology, University of Minnesota Medical School, 420 Delaware Street SE, Minneapolis, Minnesota 55455, USA
Donald Y M Leung
Timothy J Tripp
Gregory A Bohach
Cathleen A Earhart
Douglas H Ohlendorf
Patrick M Schlievert
Resource Type: Journal article
Publication Details: Biochemistry (Easton), Vol.41(47), pp.14033-14040
Publisher: United States
DOI: 10.1021/bi025977q
PMID: 12437361
ISSN: 0006-2960
eISSN: 1520-4995
Grant note: HL36611 / NHLBI NIH HHS AI51607 / NIAID NIH HHS HL36577 / NHLBI NIH HHS AR41256 / NIAMS NIH HHS AI22159 / NIAID NIH HHS HL37260 / NHLBI NIH HHS 5 T32 AI07421 / NIAID NIH HHS RR15587 / NCRR NIH HHS RR00166 / NCRR NIH HHS AI2840 / NIAID NIH HHS
Language: English
Date published: 11/26/2002
Academic Unit: Microbiology and Immunology; Internal Medicine
Record Identifier: 9984002385902771

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