Characterization of a peptide domain within the GB virus C envelope glycoprotein (E2) that inhibits HIV replication

Jinhua Xiang; James H McLinden; Thomas M Kaufman; Emma L Mohr; Nirjal Bhattarai; Qing Chang; Jack T Stapleton

doi:10.1016/j.virol.2012.04.019

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Characterization of a peptide domain within the GB virus C envelope glycoprotein (E2) that inhibits HIV replication

Journal article

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Characterization of a peptide domain within the GB virus C envelope glycoprotein (E2) that inhibits HIV replication

Jinhua Xiang, James H McLinden, Thomas M Kaufman, Emma L Mohr, Nirjal Bhattarai, Qing Chang and Jack T Stapleton

Virology (New York, N.Y.), Vol.430(1), pp.53-62

08/15/2012

DOI: 10.1016/j.virol.2012.04.019

PMCID: PMC3568392

PMID: 22608061

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Abstract

GB virus C (GBV-C) infection is associated with prolonged survival in HIV-infected cohorts, and GBV-C E2 protein inhibits HIV entry when added to CD4+ T cells. To further characterize E2 effects on HIV replication, stably transfected Jurkat cell lines expressing GBV-C E2 or control sequences were infected with HIV and replication was measured. HIV replication (all 6 isolates studied) was inhibited in all cell lines expressing a region of 17 amino acids of GBV-C E2, but not in cell lines expressing E2 without this region. In contrast, mumps and yellow fever virus replication was not inhibited by E2 protein expression. Synthetic GBV-C E2 17mer peptides did not inhibit HIV replication unless they were fused to a tat-protein-transduction-domain (TAT) for cellular uptake. These data identify the region of GBV-C E2 protein involved in HIV inhibition, and suggest that this GBV-C E2 peptide must gain entry into the cell to inhibit HIV. ► GBV-C E2 expression in a CD4+ T cell line inhibits HIV entry. ► Expression of 17 amino acids of GBV-C E2 protein inhibits HIV in a CD4+ T cell line. ► Cells expressing E2 inhibit HIV in bystander cells if there is cell-to-cell contact. ► Intracellular, but not extracellular E2 peptide inhibits HIV if it gains entry.

HIV

GBV-C

Envelope glycoprotein

Details

Title: Subtitle: Characterization of a peptide domain within the GB virus C envelope glycoprotein (E2) that inhibits HIV replication
Creators: Jinhua Xiang - Department of Internal Medicine, Iowa City Veterans Affairs Medical Center and the University of Iowa, IA 52242, USA
James H McLinden - Department of Internal Medicine, Iowa City Veterans Affairs Medical Center and the University of Iowa, IA 52242, USA
Thomas M Kaufman - Department of Internal Medicine, Iowa City Veterans Affairs Medical Center and the University of Iowa, IA 52242, USA
Emma L Mohr - Department of Internal Medicine, Iowa City Veterans Affairs Medical Center and the University of Iowa, IA 52242, USA
Nirjal Bhattarai - Department of Internal Medicine, Iowa City Veterans Affairs Medical Center and the University of Iowa, IA 52242, USA
Qing Chang - Department of Internal Medicine, Iowa City Veterans Affairs Medical Center and the University of Iowa, IA 52242, USA
Jack T Stapleton - Department of Internal Medicine, Iowa City Veterans Affairs Medical Center and the University of Iowa, IA 52242, USA
Resource Type: Journal article
Publication Details: Virology (New York, N.Y.), Vol.430(1), pp.53-62
DOI: 10.1016/j.virol.2012.04.019
PMID: 22608061
PMCID: PMC3568392
NLM abbreviation: Virology
ISSN: 0042-6822
eISSN: 1096-0341
Publisher: Elsevier Inc
Grant note: DOI: 10.13039/100000002, name: National Institutes of Health
Language: English
Date published: 08/15/2012
Academic Unit: Microbiology and Immunology; Infectious Diseases; Internal Medicine
Record Identifier: 9984094354402771

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