Journal article
Characterization of an Immunodominant Antigenic Site on GB Virus C Glycoprotein E2 That Is Involved in Cell Binding
Journal of virology, Vol.80(24), pp.12131-12140
12/2006
DOI: 10.1128/JVI.01206-06
PMCID: PMC1676310
PMID: 17035329
Abstract
GB virus type C (GBV-C) is a human flavivirus that may cause persistent infection, although most infected individuals clear viremia and develop antibodies to the envelope glycoprotein E2. To study GBV-C E2 antigenicity and cell binding, murine anti-E2 monoclonal antibodies (MAbs) were evaluated to topologically map immunogenic sites on GBV-C E2 and for the ability to detect or block recombinant E2 binding to various cell lines. Five competition groups of MAbs were identified. Groups I and II did not compete with each other. Group III competed with both groups I and II. Group IV did not compete with group I, II, or III. One MAb competed with all of the other MAbs, suggesting that the epitopes bound by these MAbs are intimately related. Individually, none of the MAbs competed extensively with polyclonal human convalescent antibody (PcAb); however, combinations of all five MAb groups completely blocked PcAb binding to E2, suggesting that the epitopes bound by these MAbs form a single, immunodominant antigenic site. Only group I and III MAbs detected purified recombinant E2 bound to cells in binding assays. In contrast, group II MAbs neutralized the binding of E2 to cells. Both PcAb and MAbs were conformation dependent, with the exception of one group II MAb (M6). M6 bound to a five-amino-acid sequence on E2 if the peptide included four C-terminal or eight N-terminal residues, suggesting that the GBV-C E2 protein contains a single immunodominant antigenic site which includes a complex epitope that is involved in specific cellular binding.
Details
- Title: Subtitle
- Characterization of an Immunodominant Antigenic Site on GB Virus C Glycoprotein E2 That Is Involved in Cell Binding
- Creators
- James H McLinden - Iowa City VA Medical Center and The University of Iowa, Iowa City, IowaThomas M Kaufman - Iowa City VA Medical Center and The University of Iowa, Iowa City, IowaJinhua Xiang - Iowa City VA Medical Center and The University of Iowa, Iowa City, IowaQing Chang - Iowa City VA Medical Center and The University of Iowa, Iowa City, IowaDonna Klinzman - Iowa City VA Medical Center and The University of Iowa, Iowa City, IowaAlfred M Engel - Iowa City VA Medical Center and The University of Iowa, Iowa City, IowaGeorg Hess - Iowa City VA Medical Center and The University of Iowa, Iowa City, IowaUrban Schmidt - Iowa City VA Medical Center and The University of Iowa, Iowa City, IowaMichael Houghton - Iowa City VA Medical Center and The University of Iowa, Iowa City, IowaJack T Stapleton - Iowa City VA Medical Center and The University of Iowa, Iowa City, Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of virology, Vol.80(24), pp.12131-12140
- DOI
- 10.1128/JVI.01206-06
- PMID
- 17035329
- PMCID
- PMC1676310
- NLM abbreviation
- J Virol
- ISSN
- 0022-538X
- eISSN
- 1098-5514
- Publisher
- American Society for Microbiology
- Language
- English
- Date published
- 12/2006
- Academic Unit
- Microbiology and Immunology; Infectious Diseases; Internal Medicine
- Record Identifier
- 9984094664502771
Metrics
16 Record Views