Journal article
Characterization of biofilms formed by Candida parapsilosis, C. metapsilosis, and C. orthopsilosis
International journal of medical microbiology, Vol.300(4), pp.265-270
2010
DOI: 10.1016/j.ijmm.2009.09.001
PMID: 19932053
Abstract
Infections due to
Candida parapsilosis have been associated with the ability of this fungus to form biofilms on indwelling medical devices. Recently,
C. parapsilosis isolates were reclassified into 3 genetically non-identical classes:
C. parapsilosis,
C. orthopsilosis, and
C. metapsilosis. Little information is available regarding the ability of these newly reclassified species to form biofilms on biomedical substrates. In this study, we characterized biofilm formation by 10 clinical isolates each of
C. parapsilosis,
C. orthopsilosis, and
C. metapsilosis. Biofilms were allowed to form on silicone elastomer discs to early (6
h) or mature (48
h) phases and quantified by tetrazolium (XTT) and dry weight assays. Surface topography and three-dimensional architecture of the biofilms were visualized using scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM), respectively. Metabolic activity assay revealed strain-dependent biofilm forming ability of the 3 species tested, while biomass determination revealed that all 3 species formed equivalent biofilms (
P>0.05 for all comparisons). SEM analyses of representative isolates of these species showed biofilms with clusters of yeast cells adherent to the catheter surface. Additionally, confocal microscopy analyses showed the presence of cells embedded in biofilms ranging in thickness between 62 and 85
μm. These results demonstrate that similar to
C. parapsilosis, the 2 newly identified
Candida species (
C. orthopsilosis and
C. metapsilosis) were able to form biofilms.
Details
- Title: Subtitle
- Characterization of biofilms formed by Candida parapsilosis, C. metapsilosis, and C. orthopsilosis
- Creators
- Ali Abdul Lattif - Center for Medical Mycology, Department of Dermatology, University Hospital's Case Medical Center, and Case Western Reserve University, 11100 Euclid Ave., Cleveland, OH 44106-502, USAPranab K Mukherjee - Center for Medical Mycology, Department of Dermatology, University Hospital's Case Medical Center, and Case Western Reserve University, 11100 Euclid Ave., Cleveland, OH 44106-502, USAJyotsna Chandra - Center for Medical Mycology, Department of Dermatology, University Hospital's Case Medical Center, and Case Western Reserve University, 11100 Euclid Ave., Cleveland, OH 44106-502, USAKim Swindell - Pediatric Infectious Diseases, Children's Hospital, Cleveland Clinic, 9500 Euclid Ave., Cleveland, OH, 44195, USAShawn R Lockhart - Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USADaniel J Diekema - University of Iowa, Internal MedicineMichael A Pfaller - University of Iowa, EpidemiologyMahmoud A Ghannoum - Center for Medical Mycology, Department of Dermatology, University Hospital's Case Medical Center, and Case Western Reserve University, 11100 Euclid Ave., Cleveland, OH 44106-502, USA
- Resource Type
- Journal article
- Publication Details
- International journal of medical microbiology, Vol.300(4), pp.265-270
- DOI
- 10.1016/j.ijmm.2009.09.001
- PMID
- 19932053
- NLM abbreviation
- Int J Med Microbiol
- ISSN
- 1438-4221
- eISSN
- 1618-0607
- Publisher
- Elsevier GmbH
- Language
- English
- Date published
- 2010
- Academic Unit
- Infectious Diseases; Epidemiology; Pathology; Internal Medicine
- Record Identifier
- 9984001178902771
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