Characterization of blue light irradiation effects on pathogenic and nonpathogenic Escherichia coli

Courtney M Abana; John R Brannon; Rebecca A Ebbott; Taryn L Dunigan; Kirsten R Guckes; Hubaida Fuseini; Jennifer Powers; Bridget R Rogers; Maria Hadjifrangiskou

doi:10.1002/mbo3.466

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Characterization of blue light irradiation effects on pathogenic and nonpathogenic Escherichia coli

Journal article

Open access

Peer reviewed

Characterization of blue light irradiation effects on pathogenic and nonpathogenic Escherichia coli

Courtney M Abana, John R Brannon, Rebecca A Ebbott, Taryn L Dunigan, Kirsten R Guckes, Hubaida Fuseini, Jennifer Powers, Bridget R Rogers and Maria Hadjifrangiskou

MicrobiologyOpen (Weinheim), Vol.6(4), e466

08/2017

DOI: 10.1002/mbo3.466

PMCID: PMC5552948

PMID: 28332311

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Abstract

Blue light irradiation (BLI) is an FDA-approved method for treating certain types of infections, like acne, and is becoming increasingly attractive as an antimicrobial strategy as the prevalence of antibiotic-resistant "superbugs" rises. However, no study has delineated the effectiveness of BLI throughout different bacterial growth phases, especially in more BLI-tolerant organisms such as Escherichia coli. While the vast majority of E. coli strains are nonpathogenic, several E. coli pathotypes exist that cause infection within and outside the gastrointestinal tract. Here, we compared the response of E. coli strains from five phylogenetic groups to BLI with a 455 nm wavelength (BLI ), using colony-forming unit and ATP measurement assays. Our results revealed that BLI is not bactericidal, but can retard E. coli growth in a manner that is dependent on culture age and strain background. This observation is critical, given that bacteria on and within mammalian hosts are found in different phases of growth.

Colony Count, Microbial

Escherichia coli - growth & development

Escherichia coli - radiation effects

Microbial Viability - radiation effects

Adenosine Triphosphate - analysis

Light

Details

Title: Subtitle: Characterization of blue light irradiation effects on pathogenic and nonpathogenic Escherichia coli
Creators: Courtney M Abana - Department of Chemical & Biomolecular Engineering, Vanderbilt University, Nashville, TN, USA
John R Brannon - Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA
Rebecca A Ebbott - Department of Biological Sciences, Vanderbilt University, Nashville, TN, USA
Taryn L Dunigan - Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, USA
Kirsten R Guckes - Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA
Hubaida Fuseini - Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA
Jennifer Powers - Vanderbilt Dermatology, Vanderbilt University Medical Center, Nashville, TN, USA
Bridget R Rogers - Department of Chemical & Biomolecular Engineering, Vanderbilt University, Nashville, TN, USA
Maria Hadjifrangiskou - Department of Urologic Surgery, Vanderbilt University Medical Center, Nashville, TN, USA
Resource Type: Journal article
Publication Details: MicrobiologyOpen (Weinheim), Vol.6(4), e466
DOI: 10.1002/mbo3.466
PMID: 28332311
PMCID: PMC5552948
NLM abbreviation: Microbiologyopen
ISSN: 2045-8827
eISSN: 2045-8827
Publisher: England
Grant note: UL1 TR000445 / NCATS NIH HHS R25 GM062459 / NIGMS NIH HHS
Language: English
Date published: 08/2017
Academic Unit: Dermatology
Record Identifier: 9984025309202771

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