Journal article
Characterization of grvA, an Antivirulence Gene on the Gifsy-2 Phage in Salmonella enterica Serovar Typhimurium
Journal of bacteriology, Vol.183(2), pp.611-620
01/01/2001
DOI: 10.1128/JB.183.2.611-620.2001
PMCID: PMC94917
PMID: 11133955
Abstract
The lambdoid phage Gifsy-2 contributes significantly to
Salmonella enterica
serovar Typhimurium virulence. The phage carries the periplasmic superoxide dismutase gene,
sodCI
, and other unidentified virulence factors. We have characterized the gene
grvA
, a single open reading frame inserted in the opposite orientation in the tail operon of the Gifsy-2 phage. Contrary to what is observed with classic virulence genes,
grvA
null mutants were more virulent than wild type as measured by intraperitoneal competition assays in mice. We have termed this effect antivirulence. Wild-type
grvA
in single copy complemented this phenotype. However,
grvA
+
on a multicopy plasmid also conferred the antivirulence phenotype. Neither a
grvA
null mutation nor the
grvA
+
plasmid conferred a growth advantage or disadvantage in laboratory media. The antivirulence phenotype conferred by the
grvA
null mutation and the
grvA
+
plasmid required wild-type
sodCI
but was independent of other virulence factors encoded on Gifsy-2. These results suggest that in a wild-type situation, GrvA decreases the pathogenicity of serovar Typhimurium in the host, most likely by affecting resistance to toxic oxygen species. These virulence phenotypes were independent of functional Gifsy-2 phage production. Our data suggest that the contribution of Gifsy-2 is a complicated sum of both positive virulence factors such as
sodCI
and antivirulence factors such as
grvA.
Details
- Title: Subtitle
- Characterization of grvA, an Antivirulence Gene on the Gifsy-2 Phage in Salmonella enterica Serovar Typhimurium
- Creators
- Theresa D. Ho - University of Illinois Urbana-ChampaignJames M. Slauch - University of Illinois Urbana-Champaign
- Resource Type
- Journal article
- Publication Details
- Journal of bacteriology, Vol.183(2), pp.611-620
- Publisher
- American Society for Microbiology
- DOI
- 10.1128/JB.183.2.611-620.2001
- PMID
- 11133955
- PMCID
- PMC94917
- ISSN
- 0021-9193
- eISSN
- 1098-5530
- Language
- English
- Date published
- 01/01/2001
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984297436902771
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