Characterization of human immunodeficiency virus type 1 replication in immature and mature dendritic cells reveals dissociable cis- and trans-infection

Chunsheng Dong; Alicia M Janas; Jian-Hua Wang; Wendy J Olson; Li Wu

doi:10.1128/JVI.01081-07

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Characterization of human immunodeficiency virus type 1 replication in immature and mature dendritic cells reveals dissociable cis- and trans-infection

Journal article

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Characterization of human immunodeficiency virus type 1 replication in immature and mature dendritic cells reveals dissociable cis- and trans-infection

Chunsheng Dong, Alicia M Janas, Jian-Hua Wang, Wendy J Olson and Li Wu

Journal of virology, Vol.81(20), pp.11352-11362

10/2007

DOI: 10.1128/JVI.01081-07

PMCID: PMC2045571

PMID: 17686876

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Abstract

Dendritic cells (DCs) transmit human immunodeficiency virus type 1 (HIV-1) to CD4(+) T cells through the trans- and cis-infection pathways; however, little is known about the relative efficiencies of these pathways and whether they are interdependent. Here we compare cis- and trans-infections of HIV-1 mediated by immature DCs (iDCs) and mature DCs (mDCs), using replication-competent and single-cycle HIV-1. Monocyte-derived iDCs were differentiated into various types of mDCs by lipopolysaccharide (LPS), tumor necrosis factor alpha (TNF-alpha), and CD40 ligand (CD40L). iDCs and CD40L-induced mDCs were susceptible to HIV-1 infection and mediated efficient viral transmission to CD4(+) T cells. Although HIV-1 cis-infection was partially restricted in TNF-alpha-induced mDCs and profoundly blocked in LPS-induced mDCs, these cells efficiently promoted HIV-1 trans-infection of CD4(+) T cells. The postentry restriction of HIV-1 infection in LPS-induced mDCs was identified at the levels of reverse transcription and postintegration, using real-time PCR quantification of viral DNA and integration. Furthermore, nucleofection of DCs with HIV-1 proviral DNA confirmed that impaired gene expression of LPS-induced mDCs was responsible for the postentry restriction of HIV-1 infection. Our results suggest that various DC subsets in vivo may differentially contribute to HIV-1 dissemination via dissociable cis- and trans-infections.

Cell Differentiation

HIV Infections

HIV-1 - pathogenicity

Virus Replication

Humans

Cells, Cultured

Dendritic Cells - pathology

CD4-Positive T-Lymphocytes - virology

Dendritic Cells - virology

Virus Integration

DNA, Viral

Details

Title: Subtitle: Characterization of human immunodeficiency virus type 1 replication in immature and mature dendritic cells reveals dissociable cis- and trans-infection
Creators: Chunsheng Dong - Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
Alicia M Janas
Jian-Hua Wang
Wendy J Olson
Li Wu
Resource Type: Journal article
Publication Details: Journal of virology, Vol.81(20), pp.11352-11362
DOI: 10.1128/JVI.01081-07
PMID: 17686876
PMCID: PMC2045571
NLM abbreviation: J Virol
ISSN: 0022-538X
eISSN: 1098-5514
Publisher: United States
Grant note: R01 AI068493 / NIAID NIH HHS R01-AI068493 / NIAID NIH HHS R01 AI068493-02 / NIAID NIH HHS
Language: English
Date published: 10/2007
Academic Unit: Microbiology and Immunology
Record Identifier: 9984001158402771

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